TY - JOUR
T1 - Highly sensitive porous carbon and metal/carbon conducting nanofiber based enzymatic biosensors for triglyceride detection
AU - Mondal, Kunal
AU - Ali, Md Azahar
AU - Singh, Chandan
AU - Sumana, Gajjala
AU - Malhotra, Bansi D.
AU - Sharma, Ashutosh
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017
Y1 - 2017
N2 - Electrospinning was employed to synthesize a Ag nanoparticle (NP)-impregnated partially aligned and free-standing carbon nanofibers (CNFs) mat using a polyacrylonitrile (PAN) and silver nitrate blend followed by carbonization. Pyrolyzation of the PAN/AgNO3blend produced the nanoporous CNFs, and the Ag NPs were grown within the CNFs via thermal decomposition of AgNO3. The fiber diameters of the synthesized CNFs ranged from 130 to 190 nm, and the size of the impregnated Ag NPs was ∼30 nm. The presence of the Ag NPs enhanced the electrical conductivity and promoted graphitization of the CNFs via the templating effect of the Ag NPs. These synthesized CNFs and AgCNFs nanocomposite were electrophoretically deposited onto indium tin oxide electrodes for detection of triglyceride molecules. Oxygen plasma treatment of the CNFs and AgCNFs surfaces resulted in enhanced loading of lipase and glycerol dehydrogenase bienzymes. The AgCNFs nanocomposite exhibited faster electron transfer than the CNFs, as corroborated by electrochemical impedance spectroscopy and cyclic voltammetry studies. Covalent functionalization via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide coupling chemistry on a nanoporous CNFs surface led to higher stability of the fabricated biosensor toward triglyceride detection. The sensitivity was four-fold higher for the AgCNFs (1.232 μA mg/dL−1 cm−2) bioelectrode compared with the CNFs (0.33 μA mg/dL−1 cm−2) over a wide detection range (25–500 mg/dL). These biosensors exhibited excellent selectivity, good reproducibility, and faster response (10 s). Thus, enhanced graphitization and electrical conductivity of nanoporous CNFs via incorporation of Ag NPs yields a promising platform for the detection of triglyceride (TG) biomolecules.
AB - Electrospinning was employed to synthesize a Ag nanoparticle (NP)-impregnated partially aligned and free-standing carbon nanofibers (CNFs) mat using a polyacrylonitrile (PAN) and silver nitrate blend followed by carbonization. Pyrolyzation of the PAN/AgNO3blend produced the nanoporous CNFs, and the Ag NPs were grown within the CNFs via thermal decomposition of AgNO3. The fiber diameters of the synthesized CNFs ranged from 130 to 190 nm, and the size of the impregnated Ag NPs was ∼30 nm. The presence of the Ag NPs enhanced the electrical conductivity and promoted graphitization of the CNFs via the templating effect of the Ag NPs. These synthesized CNFs and AgCNFs nanocomposite were electrophoretically deposited onto indium tin oxide electrodes for detection of triglyceride molecules. Oxygen plasma treatment of the CNFs and AgCNFs surfaces resulted in enhanced loading of lipase and glycerol dehydrogenase bienzymes. The AgCNFs nanocomposite exhibited faster electron transfer than the CNFs, as corroborated by electrochemical impedance spectroscopy and cyclic voltammetry studies. Covalent functionalization via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide coupling chemistry on a nanoporous CNFs surface led to higher stability of the fabricated biosensor toward triglyceride detection. The sensitivity was four-fold higher for the AgCNFs (1.232 μA mg/dL−1 cm−2) bioelectrode compared with the CNFs (0.33 μA mg/dL−1 cm−2) over a wide detection range (25–500 mg/dL). These biosensors exhibited excellent selectivity, good reproducibility, and faster response (10 s). Thus, enhanced graphitization and electrical conductivity of nanoporous CNFs via incorporation of Ag NPs yields a promising platform for the detection of triglyceride (TG) biomolecules.
KW - Biosensor
KW - Carbon nanofibers
KW - Electrochemical technique
KW - Electrospun nanofibers
KW - Silver-carbon composite nanofibers
KW - Triglyceride
UR - http://www.scopus.com/inward/record.url?scp=85013219037&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2017.02.050
DO - 10.1016/j.snb.2017.02.050
M3 - Article
AN - SCOPUS:85013219037
SN - 0925-4005
VL - 246
SP - 202
EP - 214
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -