TY - JOUR
T1 - High-resolution neutron spectroscopy on protein solution samples
AU - Grimaldo, Marco
AU - Roosen-Runge, Felix
AU - Jalarvo, Niina
AU - Zamponi, Michaela
AU - Zanini, Fabio
AU - Hennig, Marcus
AU - Zhang, Fajun
AU - Schreiber, Frank
AU - Seydel, Tilo
N1 - Publisher Copyright:
© 2015 Owned by the authors, published by EDP Sciences.
PY - 2015/1/23
Y1 - 2015/1/23
N2 - Proteins in solution move subject to a complex superposition of global translational and rotational diffusion as well as internal relaxations covering a wide range of time scales. With the advent of new high-flux neutron spectrometers in combination with enhanced analysis frameworks it has become possible to separate these different contributions. We discuss new approaches to the analysis by presenting example spectra and fits from data recorded on the backscattering spectrometers IN16, IN16B, and BASIS on the same protein solution sample. We illustrate the separation of the rotational and translational diffusion contribution, the accurate treatment of the solvent contribution, and the extraction of information on internal fluctuations. We also exemplify the progress made in passing from second- to third-generation backscattering spectrometers.
AB - Proteins in solution move subject to a complex superposition of global translational and rotational diffusion as well as internal relaxations covering a wide range of time scales. With the advent of new high-flux neutron spectrometers in combination with enhanced analysis frameworks it has become possible to separate these different contributions. We discuss new approaches to the analysis by presenting example spectra and fits from data recorded on the backscattering spectrometers IN16, IN16B, and BASIS on the same protein solution sample. We illustrate the separation of the rotational and translational diffusion contribution, the accurate treatment of the solvent contribution, and the extraction of information on internal fluctuations. We also exemplify the progress made in passing from second- to third-generation backscattering spectrometers.
UR - http://www.scopus.com/inward/record.url?scp=84921820321&partnerID=8YFLogxK
U2 - 10.1051/epjconf/20158302005
DO - 10.1051/epjconf/20158302005
M3 - Conference article
AN - SCOPUS:84921820321
SN - 2101-6275
VL - 83
JO - EPJ Web of Conferences
JF - EPJ Web of Conferences
M1 - 02005
T2 - 11th International Conference on Quasielastic Neutron Scattering, QENS 2014 and 6th International Workshop on Inelastic Neutron Spectrometers, WINS 2014
Y2 - 11 May 2014 through 16 May 2014
ER -