Hemicellulose characterization of deuterated switchgrass

Samarthya Bhagia; Yunqiao Pu; Barbara R. Evans; Brian H. Davison; Arthur J. Ragauskas

doi:10.1016/j.biortech.2018.08.034

Hemicellulose characterization of deuterated switchgrass

Samarthya Bhagia, Yunqiao Pu, Barbara R. Evans, Brian H. Davison, Arthur J. Ragauskas

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28 Scopus citations

Abstract

This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H₂O medium (protiated) or 50% D₂O medium (deuterated) through compositional analysis, GPC, FTIR, ¹³C and ¹H/¹³C HSQC NMR. 4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass. Both had comparable arabinose to xylose ratio (0.25) and molecular weight (47–50 kDa). Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.

Original language	English
Pages (from-to)	567-570
Number of pages	4
Journal	Bioresource Technology
Volume	269
DOIs	https://doi.org/10.1016/j.biortech.2018.08.034
State	Published - Dec 2018

Funding

This research was funded by the U. S. Department of Energy Office of Science through the Genomic Science Program, Office of Biological and Environmental Research, under contract FWP ERKP752. Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the U. S. Department of Energy under Contract DE-AC05-00OR22725.

Keywords

Deuterated hemicellulose
Deuterated switchgrass
Glucuronoarabinoxylan

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10.1016/j.biortech.2018.08.034

Cite this

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title = "Hemicellulose characterization of deuterated switchgrass",

abstract = "This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR. 4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass. Both had comparable arabinose to xylose ratio (0.25) and molecular weight (47–50 kDa). Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.",

keywords = "Deuterated hemicellulose, Deuterated switchgrass, Glucuronoarabinoxylan",

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AU - Bhagia, Samarthya

AU - Pu, Yunqiao

AU - Evans, Barbara R.

AU - Davison, Brian H.

AU - Ragauskas, Arthur J.

PY - 2018/12

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AB - This work describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H2O medium (protiated) or 50% D2O medium (deuterated) through compositional analysis, GPC, FTIR, 13C and 1H/13C HSQC NMR. 4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass. Both had comparable arabinose to xylose ratio (0.25) and molecular weight (47–50 kDa). Structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.

KW - Deuterated hemicellulose

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Hemicellulose characterization of deuterated switchgrass

Abstract

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Keywords

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