Glucose biosensing using an enzyme-coated microcantilever

A. Subramanian; P. I. Oden; S. J. Kennel; K. B. Jacobson; R. J. Warmack; T. Thundat; M. J. Doktycz

doi:10.1063/1.1492308

Glucose biosensing using an enzyme-coated microcantilever

A. Subramanian, P. I. Oden, S. J. Kennel, K. B. Jacobson, R. J. Warmack, T. Thundat, M. J. Doktycz

Biosciences Division

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

A microcantilever-based biosensor is described. The enzyme glucose oxidase was immobilized on a micromachined silicon cantilever containing a gold coating, such as those used for atomic force microscopy. Specific, quantifiable deflection of the derivatized cantilevers was observed in the presence of the appropriate analyte. An analysis of the reaction energetics and the expected thermal response of the cantilever indicates that cantilever deflection is not simply a result of reaction-generated heat. This deflection appears to result from surface induced stresses. The combination of a highly specific enzyme and the microcantilever platform provides a unique approach for quantifying enzyme substrates without the complication of sample labeling.

Original language	English
Pages (from-to)	385-387
Number of pages	3
Journal	Applied Physics Letters
Volume	81
Issue number	2
DOIs	https://doi.org/10.1063/1.1492308
State	Published - Jul 8 2002

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title = "Glucose biosensing using an enzyme-coated microcantilever",

abstract = "A microcantilever-based biosensor is described. The enzyme glucose oxidase was immobilized on a micromachined silicon cantilever containing a gold coating, such as those used for atomic force microscopy. Specific, quantifiable deflection of the derivatized cantilevers was observed in the presence of the appropriate analyte. An analysis of the reaction energetics and the expected thermal response of the cantilever indicates that cantilever deflection is not simply a result of reaction-generated heat. This deflection appears to result from surface induced stresses. The combination of a highly specific enzyme and the microcantilever platform provides a unique approach for quantifying enzyme substrates without the complication of sample labeling.",

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T1 - Glucose biosensing using an enzyme-coated microcantilever

AU - Subramanian, A.

AU - Oden, P. I.

AU - Kennel, S. J.

AU - Jacobson, K. B.

AU - Warmack, R. J.

AU - Thundat, T.

AU - Doktycz, M. J.

PY - 2002/7/8

Y1 - 2002/7/8

N2 - A microcantilever-based biosensor is described. The enzyme glucose oxidase was immobilized on a micromachined silicon cantilever containing a gold coating, such as those used for atomic force microscopy. Specific, quantifiable deflection of the derivatized cantilevers was observed in the presence of the appropriate analyte. An analysis of the reaction energetics and the expected thermal response of the cantilever indicates that cantilever deflection is not simply a result of reaction-generated heat. This deflection appears to result from surface induced stresses. The combination of a highly specific enzyme and the microcantilever platform provides a unique approach for quantifying enzyme substrates without the complication of sample labeling.

AB - A microcantilever-based biosensor is described. The enzyme glucose oxidase was immobilized on a micromachined silicon cantilever containing a gold coating, such as those used for atomic force microscopy. Specific, quantifiable deflection of the derivatized cantilevers was observed in the presence of the appropriate analyte. An analysis of the reaction energetics and the expected thermal response of the cantilever indicates that cantilever deflection is not simply a result of reaction-generated heat. This deflection appears to result from surface induced stresses. The combination of a highly specific enzyme and the microcantilever platform provides a unique approach for quantifying enzyme substrates without the complication of sample labeling.

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DO - 10.1063/1.1492308

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VL - 81

SP - 385

EP - 387

JO - Applied Physics Letters

JF - Applied Physics Letters

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Glucose biosensing using an enzyme-coated microcantilever

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