TY - GEN
T1 - Fully automated segmentation and characterization of the dendritic trees of retinal horizontal neurons
AU - Kerekes, Ryan A.
AU - Gleason, Shaun S.
AU - Martins, Rodrigo A.P.
AU - Dyer, Michael
PY - 2010
Y1 - 2010
N2 - We introduce a new fully automated method for segmenting and characterizing the dendritic tree of neurons in confocal image stacks. Our method is aimed at wide-field-of-view, low-resolution imagery of retinal neurons in which dendrites can be intertwined and difficult to follow. The approach is based on 3-D skeletonization and includes a method for automatically determining an appropriate global threshold as well as a soma detection algorithm. We provide the details of the algorithm and a qualitative performance comparison against a commercially available neurite tracing software package, showing that a segmentation produced by our method more closely matches the ground-truth segmentation.
AB - We introduce a new fully automated method for segmenting and characterizing the dendritic tree of neurons in confocal image stacks. Our method is aimed at wide-field-of-view, low-resolution imagery of retinal neurons in which dendrites can be intertwined and difficult to follow. The approach is based on 3-D skeletonization and includes a method for automatically determining an appropriate global threshold as well as a soma detection algorithm. We provide the details of the algorithm and a qualitative performance comparison against a commercially available neurite tracing software package, showing that a segmentation produced by our method more closely matches the ground-truth segmentation.
UR - http://www.scopus.com/inward/record.url?scp=77955643810&partnerID=8YFLogxK
U2 - 10.1109/BSEC.2010.5510843
DO - 10.1109/BSEC.2010.5510843
M3 - Conference contribution
AN - SCOPUS:77955643810
SN - 9781424467143
T3 - Proceedings of the 2010 Biomedical Science and Engineering Conference, BSEC 2010: Biomedical Research and Analysis in Neuroscience, BRAiN
BT - Proceedings of the 2010 Biomedical Science and Engineering Conference, BSEC 2010
T2 - 2010 Biomedical Science and Engineering Conference, BSEC 2010: Biomedical Research and Analysis in Neuroscience, BRAiN
Y2 - 25 May 2010 through 26 May 2010
ER -