Fluorescence energy transfer efficiency in labeled yeast cytochrome c: A rapid screen for ion biocompatibility in aqueous ionic liquids

Sheila N. Baker; Hua Zhao; Siddharth Pandey; William T. Heller; Frank V. Bright; Gary A. Baker

doi:10.1039/c0cp02345k

Fluorescence energy transfer efficiency in labeled yeast cytochrome c: A rapid screen for ion biocompatibility in aqueous ionic liquids

Sheila N. Baker, Hua Zhao, Siddharth Pandey, William T. Heller, Frank V. Bright, Gary A. Baker

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Abstract

A fluorescence energy transfer "de-quenching" assay was implemented to follow the equilibrium unfolding behaviour of site-specific tetramethylrhodamine-labelled yeast cytochrome c in aqueous ionic liquid solutions; additionally, this approach offers the prospect of naked eye screening for biocompatible ion combinations in hydrated ionic liquids.

Original language	English
Pages (from-to)	3642-3644
Number of pages	3
Journal	Physical Chemistry Chemical Physics
Volume	13
Issue number	9
DOIs	https://doi.org/10.1039/c0cp02345k
State	Published - Mar 7 2011

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abstract = "A fluorescence energy transfer {"}de-quenching{"} assay was implemented to follow the equilibrium unfolding behaviour of site-specific tetramethylrhodamine-labelled yeast cytochrome c in aqueous ionic liquid solutions; additionally, this approach offers the prospect of naked eye screening for biocompatible ion combinations in hydrated ionic liquids.",

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AU - Baker, Sheila N.

AU - Zhao, Hua

AU - Pandey, Siddharth

AU - Heller, William T.

AU - Bright, Frank V.

AU - Baker, Gary A.

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AB - A fluorescence energy transfer "de-quenching" assay was implemented to follow the equilibrium unfolding behaviour of site-specific tetramethylrhodamine-labelled yeast cytochrome c in aqueous ionic liquid solutions; additionally, this approach offers the prospect of naked eye screening for biocompatible ion combinations in hydrated ionic liquids.

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U2 - 10.1039/c0cp02345k

DO - 10.1039/c0cp02345k

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EP - 3644

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

IS - 9

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Fluorescence energy transfer efficiency in labeled yeast cytochrome c: A rapid screen for ion biocompatibility in aqueous ionic liquids

Abstract

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