Fluorescence-based Lab-on-Chip spot design for improved signal detection

Thiago Bassani, Philipe Dias, Gilberto Branco, Wilson J. Da Silva, Hugo Vieira Neto, Fabio K. Schneider

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

This work presents a new perspective for fluorescence signal detection using specific optics on Lab-on-Chip devices. An apparatus was designed and implemented in order to assess the performance of a fluorescence technique using different detection spot configurations, using the chip itself as a waveguide for illumination. The experiments conducted investigate the influence of the dimensions - diameter and height - of the spot on the amplitude of the detection output signal. Results show that the configuration of optical interfaces must be considered in order to improve detection output, or to be able to detect less fluorophore molecules in the spot.

Original languageEnglish
Title of host publication2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012
Pages543-546
Number of pages4
DOIs
StatePublished - 2012
Externally publishedYes
Event34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2012 - San Diego, CA, United States
Duration: Aug 28 2012Sep 1 2012

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)1557-170X

Conference

Conference34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2012
Country/TerritoryUnited States
CitySan Diego, CA
Period08/28/1209/1/12

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