Fluid shear stress enhances proliferation of breast cancer cells via downregulation of the c-subunit of the F1FO ATP synthase

Han A. Park; Spenser R. Brown; Joseph Jansen; Tracie Dunn; Madison Scott; Nelli Mnatsakanyan; Elizabeth A. Jonas; Yonghyun Kim

doi:10.1016/j.bbrc.2022.09.084

Fluid shear stress enhances proliferation of breast cancer cells via downregulation of the c-subunit of the F₁F_O ATP synthase

Han A. Park, Spenser R. Brown, Joseph Jansen, Tracie Dunn, Madison Scott, Nelli Mnatsakanyan, Elizabeth A. Jonas, Yonghyun Kim

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The presence of circulating cancer cells in the bloodstream is positively correlated with metastasis. We hypothesize that fluid shear stress (FSS) occurring during circulation alters mitochondrial function, enhancing metastatic behaviors of cancer cells. MCF7 and MDA-MB-231 human breast cancer cells subjected to FSS exponentially increased proliferation. Notably, FSS-treated cells consumed more oxygen but were resistant to uncoupler-mediated ATP loss. We found that exposure to FSS downregulated the F₁F_O ATP synthase c-subunit and overexpression of the c-subunit arrested cancer cell migration. Approaches that regulate c-subunit abundance may reduce the likelihood of breast cancer metastasis.

Original language	English
Pages (from-to)	173-180
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	632
DOIs	https://doi.org/10.1016/j.bbrc.2022.09.084
State	Published - Dec 3 2022
Externally published	Yes

Keywords

Breast cancer
FF ATP synthase
Fluid shear stress
Mitochondria

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.bbrc.2022.09.084

Cite this

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title = "Fluid shear stress enhances proliferation of breast cancer cells via downregulation of the c-subunit of the F1FO ATP synthase",

abstract = "The presence of circulating cancer cells in the bloodstream is positively correlated with metastasis. We hypothesize that fluid shear stress (FSS) occurring during circulation alters mitochondrial function, enhancing metastatic behaviors of cancer cells. MCF7 and MDA-MB-231 human breast cancer cells subjected to FSS exponentially increased proliferation. Notably, FSS-treated cells consumed more oxygen but were resistant to uncoupler-mediated ATP loss. We found that exposure to FSS downregulated the F1FO ATP synthase c-subunit and overexpression of the c-subunit arrested cancer cell migration. Approaches that regulate c-subunit abundance may reduce the likelihood of breast cancer metastasis.",

keywords = "Breast cancer, FF ATP synthase, Fluid shear stress, Mitochondria",

author = "Park, {Han A.} and Brown, {Spenser R.} and Joseph Jansen and Tracie Dunn and Madison Scott and Nelli Mnatsakanyan and Jonas, {Elizabeth A.} and Yonghyun Kim",

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doi = "10.1016/j.bbrc.2022.09.084",

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T1 - Fluid shear stress enhances proliferation of breast cancer cells via downregulation of the c-subunit of the F1FO ATP synthase

AU - Park, Han A.

AU - Brown, Spenser R.

AU - Jansen, Joseph

AU - Dunn, Tracie

AU - Scott, Madison

AU - Mnatsakanyan, Nelli

AU - Jonas, Elizabeth A.

AU - Kim, Yonghyun

PY - 2022/12/3

Y1 - 2022/12/3

N2 - The presence of circulating cancer cells in the bloodstream is positively correlated with metastasis. We hypothesize that fluid shear stress (FSS) occurring during circulation alters mitochondrial function, enhancing metastatic behaviors of cancer cells. MCF7 and MDA-MB-231 human breast cancer cells subjected to FSS exponentially increased proliferation. Notably, FSS-treated cells consumed more oxygen but were resistant to uncoupler-mediated ATP loss. We found that exposure to FSS downregulated the F1FO ATP synthase c-subunit and overexpression of the c-subunit arrested cancer cell migration. Approaches that regulate c-subunit abundance may reduce the likelihood of breast cancer metastasis.

AB - The presence of circulating cancer cells in the bloodstream is positively correlated with metastasis. We hypothesize that fluid shear stress (FSS) occurring during circulation alters mitochondrial function, enhancing metastatic behaviors of cancer cells. MCF7 and MDA-MB-231 human breast cancer cells subjected to FSS exponentially increased proliferation. Notably, FSS-treated cells consumed more oxygen but were resistant to uncoupler-mediated ATP loss. We found that exposure to FSS downregulated the F1FO ATP synthase c-subunit and overexpression of the c-subunit arrested cancer cell migration. Approaches that regulate c-subunit abundance may reduce the likelihood of breast cancer metastasis.

KW - Breast cancer

KW - FF ATP synthase

KW - Fluid shear stress

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