TY - JOUR
T1 - Fermentation, hydrogen, and sulfur metabolism in multiple uncultivated bacterial phyla
AU - Wrighton, Kelly C.
AU - Thomas, Brian C.
AU - Sharon, Itai
AU - Miller, Christopher S.
AU - Castelle, Cindy J.
AU - VerBerkmoes, Nathan C.
AU - Wilkins, Michael J.
AU - Hettich, Robert L.
AU - Lipton, Mary S.
AU - Williams, Kenneth H.
AU - Long, Philip E.
AU - Banfield, Jillian F.
PY - 2012/9/28
Y1 - 2012/9/28
N2 - BD1-5, OP11, and OD1 bacteria have been widely detected in anaerobic environments, but their metabolisms remain unclear owing to lack of cultivated representatives and minimal genomic sampling. We uncovered metabolic characteristics for members of these phyla, and a new lineage, PER, via cultivation-independent recovery of 49 partial to near-complete genomes from an acetate-amended aquifer. All organisms were nonrespiring anaerobes predicted to ferment. Three augment fermentation with archaeal-like hybrid type II/III ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) that couples adenosine monophosphate salvage with CO2fixation, a pathway not previously described in Bacteria. Members of OD1 reduce sulfur and may pump protons using archaeal-type hydrogenases. For six organisms, the UGA stop codon is translated as tryptophan. All bacteria studied here may play previously unrecognized roles in hydrogen production, sulfur cycling, and fermentation of refractory sedimentary carbon.
AB - BD1-5, OP11, and OD1 bacteria have been widely detected in anaerobic environments, but their metabolisms remain unclear owing to lack of cultivated representatives and minimal genomic sampling. We uncovered metabolic characteristics for members of these phyla, and a new lineage, PER, via cultivation-independent recovery of 49 partial to near-complete genomes from an acetate-amended aquifer. All organisms were nonrespiring anaerobes predicted to ferment. Three augment fermentation with archaeal-like hybrid type II/III ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) that couples adenosine monophosphate salvage with CO2fixation, a pathway not previously described in Bacteria. Members of OD1 reduce sulfur and may pump protons using archaeal-type hydrogenases. For six organisms, the UGA stop codon is translated as tryptophan. All bacteria studied here may play previously unrecognized roles in hydrogen production, sulfur cycling, and fermentation of refractory sedimentary carbon.
UR - https://www.scopus.com/pages/publications/84866772472
U2 - 10.1126/science.1224041
DO - 10.1126/science.1224041
M3 - Article
AN - SCOPUS:84866772472
SN - 0036-8075
VL - 337
SP - 1661
EP - 1665
JO - Science
JF - Science
IS - 6102
ER -