Extraction and analysis of neuron firing signals from deep cortical video microscopy

Ryan Kerekes, Jay Blundon

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We introduce a method for extracting and analyzing neuronal activity time signals from video o. The cortex of a live animal. The signals correspond t. The firing activity of individual cortical neurons. Activity signals are based o. The changing fluorescence of calcium indicators i. The cells over time. We propose a cell segmentation method that relies on a user-specified center point, from whic. The signal extraction method proceeds. A stabilization approach is used to reduce tissue motion i. The video. The extracted signal i. Then processed to flatte. The baseline and detect action potentials. We show results from applyin. The method to a cortical video of a live mouse.

Original languageEnglish
Title of host publicationProceedings of the 2014 Biomedical Sciences and Engineering Conference - 5th Annual ORNL Biomedical Sciences and Engineering Conference
Subtitle of host publicationCollaborative Biomedical Innovations - The Multi-Scale Brain: Spanning Molecular, Cellular, Systems, Cognitive, Behavioral, and Clinical Neuroscience, BSEC 2014
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781479941599
DOIs
StatePublished - Jul 29 2014
Event5th Annual ORNL Biomedical Sciences and Engineering Conference: Collaborative Biomedical Innovations, BSEC 2014 - Oak Ridge, United States
Duration: May 6 2014May 8 2014

Publication series

NameProceedings of the 2014 Biomedical Sciences and Engineering Conference - 5th Annual ORNL Biomedical Sciences and Engineering Conference: Collaborative Biomedical Innovations - The Multi-Scale Brain: Spanning Molecular, Cellular, Systems, Cognitive, Behavioral, and Clinical Neuroscience, BSEC 2014

Conference

Conference5th Annual ORNL Biomedical Sciences and Engineering Conference: Collaborative Biomedical Innovations, BSEC 2014
Country/TerritoryUnited States
CityOak Ridge
Period05/6/1405/8/14

Keywords

  • calcium fluorescence
  • neuron activity
  • signal extraction
  • signal processing

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