Expression profiles of 2 phosphate starvation-inducible phosphocholine/phosphoethanolamine phosphatases, PECP1 and PS2, in arabidopsis

Artik Elisa Angkawijaya; Anh H. Ngo; Van C. Nguyen; Farrel Gunawan; Yuki Nakamura

doi:10.3389/fpls.2019.00662

Expression profiles of 2 phosphate starvation-inducible phosphocholine/phosphoethanolamine phosphatases, PECP1 and PS2, in arabidopsis

Artik Elisa Angkawijaya, Anh H. Ngo, Van C. Nguyen, Farrel Gunawan, Yuki Nakamura

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Phosphorus is essential for plant viability. Phosphate-starved plants trigger membrane lipid remodeling to replace membrane phospholipids by non-phosphorus galactolipids presumably to acquire scarce phosphate source. Phosphoethanolamine/phosphocholine phosphatase 1 (PECP1) and phosphate starvation-induced gene 2 (PS2) belong to an emerging class of phosphatase induced by phosphate starvation and dephosphorylates phosphocholine and phosphoethanolamine (PEtn) in vivo. However, detailed spatiotemporal expression pattern as well as subcellular localization has not been investigated yet. Here, by constructing transgenic plants harboring functional translational promoter–reporter fusion system, we showed the expression pattern of PECP1 and PS2 in different tissues and in response to phosphate starvation. Besides, the Venus fluorescent reporter revealed that both are localized at the ER. Characterization of transgenic plants that overexpress PECP1 or PS2 showed that their activity toward PEtn may be different in vivo. We suggest that PECP1 and PS2 are ER-localized phosphatases that show similar expression pattern yet have a distinct substrate specificity in vivo.

Original language	English
Article number	662
Journal	Frontiers in Plant Science
Volume	10
DOIs	https://doi.org/10.3389/fpls.2019.00662
State	Published - May 31 2019
Externally published	Yes

Funding

The authors thank the staffs at Institute of Plant and Microbial Biology, Academia Sinica; Yu-Ching Wu (Small Molecule Metabolomics Core Lab) for assistance with metabolite analysis, and Yu-chi Liu and Pannada Kungwon for molecular cloning. Funding. This research was supported by the Ministry of Science and Technology, Taiwan (Grant ID 106-2628-B-001-002 and 107-2628-B-001-001 to YN) and core research budget provided by Academia Sinica (YN). YN is supported by EMBO Young Investigator Program. This research was supported by the Ministry of Science and Technology, Taiwan (Grant ID 106-2628-B-001-002 and 107-2628-B-001-001 to YN) and core research budget provided

Keywords

Arabidopsis thaliana
Membrane lipid remodeling
Phosphatase
Phosphate starvation
Phospholipids

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@article{c7f31fc42dc74bc2b5d9477f6efb4810,

title = "Expression profiles of 2 phosphate starvation-inducible phosphocholine/phosphoethanolamine phosphatases, PECP1 and PS2, in arabidopsis",

abstract = "Phosphorus is essential for plant viability. Phosphate-starved plants trigger membrane lipid remodeling to replace membrane phospholipids by non-phosphorus galactolipids presumably to acquire scarce phosphate source. Phosphoethanolamine/phosphocholine phosphatase 1 (PECP1) and phosphate starvation-induced gene 2 (PS2) belong to an emerging class of phosphatase induced by phosphate starvation and dephosphorylates phosphocholine and phosphoethanolamine (PEtn) in vivo. However, detailed spatiotemporal expression pattern as well as subcellular localization has not been investigated yet. Here, by constructing transgenic plants harboring functional translational promoter–reporter fusion system, we showed the expression pattern of PECP1 and PS2 in different tissues and in response to phosphate starvation. Besides, the Venus fluorescent reporter revealed that both are localized at the ER. Characterization of transgenic plants that overexpress PECP1 or PS2 showed that their activity toward PEtn may be different in vivo. We suggest that PECP1 and PS2 are ER-localized phosphatases that show similar expression pattern yet have a distinct substrate specificity in vivo.",

keywords = "Arabidopsis thaliana, Membrane lipid remodeling, Phosphatase, Phosphate starvation, Phospholipids",

author = "Angkawijaya, \{Artik Elisa\} and Ngo, \{Anh H.\} and Nguyen, \{Van C.\} and Farrel Gunawan and Yuki Nakamura",

note = "Publisher Copyright: {\textcopyright} 2019 Angkawijaya, Ngo, Nguyen, Gunawan and Nakamura.",

year = "2019",

month = may,

day = "31",

doi = "10.3389/fpls.2019.00662",

language = "English",

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journal = "Frontiers in Plant Science",

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T1 - Expression profiles of 2 phosphate starvation-inducible phosphocholine/phosphoethanolamine phosphatases, PECP1 and PS2, in arabidopsis

AU - Angkawijaya, Artik Elisa

AU - Ngo, Anh H.

AU - Nguyen, Van C.

AU - Gunawan, Farrel

AU - Nakamura, Yuki

PY - 2019/5/31

Y1 - 2019/5/31

N2 - Phosphorus is essential for plant viability. Phosphate-starved plants trigger membrane lipid remodeling to replace membrane phospholipids by non-phosphorus galactolipids presumably to acquire scarce phosphate source. Phosphoethanolamine/phosphocholine phosphatase 1 (PECP1) and phosphate starvation-induced gene 2 (PS2) belong to an emerging class of phosphatase induced by phosphate starvation and dephosphorylates phosphocholine and phosphoethanolamine (PEtn) in vivo. However, detailed spatiotemporal expression pattern as well as subcellular localization has not been investigated yet. Here, by constructing transgenic plants harboring functional translational promoter–reporter fusion system, we showed the expression pattern of PECP1 and PS2 in different tissues and in response to phosphate starvation. Besides, the Venus fluorescent reporter revealed that both are localized at the ER. Characterization of transgenic plants that overexpress PECP1 or PS2 showed that their activity toward PEtn may be different in vivo. We suggest that PECP1 and PS2 are ER-localized phosphatases that show similar expression pattern yet have a distinct substrate specificity in vivo.

AB - Phosphorus is essential for plant viability. Phosphate-starved plants trigger membrane lipid remodeling to replace membrane phospholipids by non-phosphorus galactolipids presumably to acquire scarce phosphate source. Phosphoethanolamine/phosphocholine phosphatase 1 (PECP1) and phosphate starvation-induced gene 2 (PS2) belong to an emerging class of phosphatase induced by phosphate starvation and dephosphorylates phosphocholine and phosphoethanolamine (PEtn) in vivo. However, detailed spatiotemporal expression pattern as well as subcellular localization has not been investigated yet. Here, by constructing transgenic plants harboring functional translational promoter–reporter fusion system, we showed the expression pattern of PECP1 and PS2 in different tissues and in response to phosphate starvation. Besides, the Venus fluorescent reporter revealed that both are localized at the ER. Characterization of transgenic plants that overexpress PECP1 or PS2 showed that their activity toward PEtn may be different in vivo. We suggest that PECP1 and PS2 are ER-localized phosphatases that show similar expression pattern yet have a distinct substrate specificity in vivo.

KW - Arabidopsis thaliana

KW - Membrane lipid remodeling

KW - Phosphatase

KW - Phosphate starvation

KW - Phospholipids

UR - https://www.scopus.com/pages/publications/85068437863

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DO - 10.3389/fpls.2019.00662

M3 - Article

AN - SCOPUS:85068437863

SN - 1664-462X

VL - 10

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

M1 - 662

ER -

Expression profiles of 2 phosphate starvation-inducible phosphocholine/phosphoethanolamine phosphatases, PECP1 and PS2, in arabidopsis

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Keywords

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