TY - JOUR
T1 - Expression analysis of the AtMLO gene family encoding plant-specific seven-transmembrane domain proteins
AU - Chen, Zhongying
AU - Hartmann, H. Andreas
AU - Wu, Ming Jing
AU - Friedman, Erin J.
AU - Chen, Jin Gui
AU - Pulley, Matthew
AU - Schulze-Lefert, Paul
AU - Panstruga, Ralph
AU - Jones, Alan M.
PY - 2006/3
Y1 - 2006/3
N2 - The Arabidopsis (Arabidopsis thaliana) genome contains 15 genes encoding protein homologs of the barley mildew resistance locus o (MLO) protein biochemically shown to have a seven-transmembrane domain topology and localize to the plasma membrane. Towards elucidating the functions of MLOs, the largest family of seven-transmembrane domain proteins specific to plants, we comprehensively determined AtMLO gene expression patterns by a combination of experimental and in silico studies. Experimentation comprised analyses of transgenic Arabidopsis lines bearing promoter::β-glucuronidase (GUS) transcriptional fusions as well as semi-quantitative determination of transcripts by reverse transcription coupled to polymerase chain reaction (RT-PCR). These results were combined with information extracted from public gene profiling databases, and compared to the expression patterns of genes encoding the heterotrimeric G-protein subunits. We found that each AtMLO gene has a unique expression pattern and is regulated differently by a variety of biotic and/or abiotic stimuli, suggesting that AtMLO proteins function in diverse developmental and response processes. The expression of several phylogenetically closely-related AtMLO genes showed similar or overlapping tissue specificity and analogous responsiveness to external stimuli, suggesting functional redundancy, co-function, or antagonistic function(s).
AB - The Arabidopsis (Arabidopsis thaliana) genome contains 15 genes encoding protein homologs of the barley mildew resistance locus o (MLO) protein biochemically shown to have a seven-transmembrane domain topology and localize to the plasma membrane. Towards elucidating the functions of MLOs, the largest family of seven-transmembrane domain proteins specific to plants, we comprehensively determined AtMLO gene expression patterns by a combination of experimental and in silico studies. Experimentation comprised analyses of transgenic Arabidopsis lines bearing promoter::β-glucuronidase (GUS) transcriptional fusions as well as semi-quantitative determination of transcripts by reverse transcription coupled to polymerase chain reaction (RT-PCR). These results were combined with information extracted from public gene profiling databases, and compared to the expression patterns of genes encoding the heterotrimeric G-protein subunits. We found that each AtMLO gene has a unique expression pattern and is regulated differently by a variety of biotic and/or abiotic stimuli, suggesting that AtMLO proteins function in diverse developmental and response processes. The expression of several phylogenetically closely-related AtMLO genes showed similar or overlapping tissue specificity and analogous responsiveness to external stimuli, suggesting functional redundancy, co-function, or antagonistic function(s).
KW - Arabidopsis thaliana
KW - GUS staining
KW - Heterotrimeric G-protein
KW - MLO
KW - Microarray
KW - RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=33644840994&partnerID=8YFLogxK
U2 - 10.1007/s11103-005-5082-x
DO - 10.1007/s11103-005-5082-x
M3 - Article
C2 - 16525893
AN - SCOPUS:33644840994
SN - 0167-4412
VL - 60
SP - 583
EP - 597
JO - Plant Molecular Biology
JF - Plant Molecular Biology
IS - 4
ER -