EWALD: A macromolecular diffractometer for the second target station

Gloria E.O. Borgstahl; William B. O'Dell; Martin Egli; Jan F. Kern; Andrey Kovalevsky; Jiao Y.Y. Lin; Dean Myles; Mark A. Wilson; Wen Zhang; Petrus Zwart; Leighton Coates

doi:10.1063/5.0090810

EWALD: A macromolecular diffractometer for the second target station

Gloria E.O. Borgstahl, William B. O'Dell, Martin Egli, Jan F. Kern, Andrey Kovalevsky, Jiao Y.Y. Lin, Dean Myles, Mark A. Wilson, Wen Zhang, Petrus Zwart, Leighton Coates

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Revealing the positions of all the atoms in large macromolecules is powerful but only possible with neutron macromolecular crystallography (NMC). Neutrons provide a sensitive and gentle probe for the direct detection of protonation states at near-physiological temperatures and clean of artifacts caused by x rays or electrons. Currently, NMC use is restricted by the requirement for large crystal volumes even at state-of-the-art instruments such as the macromolecular neutron diffractometer at the Spallation Neutron Source. EWALD's design will break the crystal volume barrier and, thus, open the door for new types of experiments, the study of grand challenge systems, and the more routine use of NMC in biology. EWALD is a single crystal diffractometer capable of collecting data from macromolecular crystals on orders of magnitude smaller than what is currently feasible. The construction of EWALD at the Second Target Station will cause a revolution in NMC by enabling key discoveries in the biological, biomedical, and bioenergy sciences.

Original language	English
Article number	064103
Journal	Review of Scientific Instruments
Volume	93
Issue number	6
DOIs	https://doi.org/10.1063/5.0090810
State	Published - Jun 1 2022

Funding

This research used resources at the SNS and the HFIR, which are DOE Office of Science User Facilities operated by the Oak Ridge National Laboratory (ORNL). This research used resources of the Spallation Neutron Source Second Target Station Project at ORNL under Grant No. ERKC2TS. ORNL is managed by UT-Battelle LLC for DOE’s Office of Science, the single largest supporter of basic research in the physical sciences in the United States.

Funders	Funder number
Oak Ridge National Laboratory	ERKC2TS

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title = "EWALD: A macromolecular diffractometer for the second target station",

abstract = "Revealing the positions of all the atoms in large macromolecules is powerful but only possible with neutron macromolecular crystallography (NMC). Neutrons provide a sensitive and gentle probe for the direct detection of protonation states at near-physiological temperatures and clean of artifacts caused by x rays or electrons. Currently, NMC use is restricted by the requirement for large crystal volumes even at state-of-the-art instruments such as the macromolecular neutron diffractometer at the Spallation Neutron Source. EWALD's design will break the crystal volume barrier and, thus, open the door for new types of experiments, the study of grand challenge systems, and the more routine use of NMC in biology. EWALD is a single crystal diffractometer capable of collecting data from macromolecular crystals on orders of magnitude smaller than what is currently feasible. The construction of EWALD at the Second Target Station will cause a revolution in NMC by enabling key discoveries in the biological, biomedical, and bioenergy sciences.",

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AU - Borgstahl, Gloria E.O.

AU - O'Dell, William B.

AU - Egli, Martin

AU - Kern, Jan F.

AU - Kovalevsky, Andrey

AU - Lin, Jiao Y.Y.

AU - Myles, Dean

AU - Wilson, Mark A.

AU - Zhang, Wen

AU - Zwart, Petrus

AU - Coates, Leighton

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AB - Revealing the positions of all the atoms in large macromolecules is powerful but only possible with neutron macromolecular crystallography (NMC). Neutrons provide a sensitive and gentle probe for the direct detection of protonation states at near-physiological temperatures and clean of artifacts caused by x rays or electrons. Currently, NMC use is restricted by the requirement for large crystal volumes even at state-of-the-art instruments such as the macromolecular neutron diffractometer at the Spallation Neutron Source. EWALD's design will break the crystal volume barrier and, thus, open the door for new types of experiments, the study of grand challenge systems, and the more routine use of NMC in biology. EWALD is a single crystal diffractometer capable of collecting data from macromolecular crystals on orders of magnitude smaller than what is currently feasible. The construction of EWALD at the Second Target Station will cause a revolution in NMC by enabling key discoveries in the biological, biomedical, and bioenergy sciences.

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EWALD: A macromolecular diffractometer for the second target station

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