Epidermal growth factor (EGF) receptor targeted delivery of PEGylated adenovirus

Ji Won Park, Hyejung Mok, Tae Gwan Park

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

A biotin-polyethylene glycol (PEG)-epidermal growth factor (EGF) conjugate was immobilized onto the surface of avidin-modified adenovirus (ADV-Avi) via biotin-avidin interaction to deliver ADV specifically to EGF receptor over-expressing cancer cells. ADV-Avi/biotin-PEG-EGF complexes showed greatly enhanced intracellular uptake of ADV particles for an EGF receptor positive cell line (A431 cells), compared to naked or PEG alone immobilized ADV. ADV coding an exogenous GFP gene was used to quantitatively evaluate the level of GFP expression. ADV-Avi/biotin-PEG-EGF complexes also exhibited significantly increased extent of GFP expression for A431 cells, but not for MCF-7 cells (an EGF receptor deficient cell line), suggesting that retargeting of ADV to specific cells occurred by tethering of a cell-specific targeting ligand to the distal end of a PEG chain anchored onto the surface of ADV. This study demonstrates that ADV-Avi/biotin-PEG-EGF construct systems can be applied for cell-specific delivery of ADV with simultaneously reducing innate immune responses.

Original languageEnglish
Pages (from-to)769-774
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume366
Issue number3
DOIs
StatePublished - Feb 15 2008
Externally publishedYes

Funding

This study was supported by the grant of the National Cancer Center, Republic of Korea (0620240-1).

Keywords

  • Adenovirus
  • Avidin and biotin interaction
  • EGF
  • Gene delivery
  • PEG

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