Enzymatic AND logic gates operated under conditions characteristic of biomedical applications

Dmitriy Melnikov, Guinevere Strack, Jian Zhou, Joshua Ray Windmiller, Jan Halámek, Vera Bocharova, Min Chieh Chuang, Padmanabhan Santhosh, Vladimir Privman, Joseph Wang, Evgeny Katz

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Experimental and theoretical analyses of the lactate dehydrogenase and glutathione reductase based enzymatic AND logic gates in which the enzymes and their substrates serve as logic inputs are performed. These two systems are examples of the novel, previously unexplored class of biochemical logic gates that illustrate potential biomedical applications of biochemical logic. They are characterized by input concentrations at logic 0 and 1 states corresponding to normal and pathophysiological conditions. Our analysis shows that the logic gates under investigation have similar noise characteristics. Both significantly amplify random noise present in inputs; however, we establish that for realistic widths of the input noise distributions, it is still possible to differentiate between the logic 0 and 1 states of the output. This indicates that reliable detection of pathophysiological conditions is indeed possible with such enzyme logic systems.

Original languageEnglish
Pages (from-to)12166-12174
Number of pages9
JournalJournal of Physical Chemistry B
Volume114
Issue number37
DOIs
StatePublished - Sep 23 2010
Externally publishedYes

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