Enhancing transfection efficiency using polyethylene glycol grafted polyethylenimine and fusogenic peptide

Haeshin Lee; Ji Hoon Jeong; Je Hoon Lee; Tae Gwan Park

doi:10.1007/BF02931988

Enhancing transfection efficiency using polyethylene glycol grafted polyethylenimine and fusogenic peptide

Haeshin Lee, Ji Hoon Jeong, Je Hoon Lee, Tae Gwan Park

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

This study presents a new formulation method for improving DNA transfection efficiency using a fusogenic peptide and polyethylene glycol grafted polyethylenimine. Succinimidyl succinate polyethylene glycol (PEG-SSA) was conjugated with polyethylenimine (PEI). PEI is well known for a good endosomal escaping and DNA condensing agent. The positively charged synthetic fusogenic peptide, KALA, was coated on the negatively charged PEG-g-PEI/DNA and PEI/DNA complexes. The KALA/PEI/DNA complexes exhibited aggregation behavior at higher KALA coating amounts with an effective diameter of around 1,000 nm. However, the KALA/PEG-g-PEI/DNA complexes were 100-300 nm in size with a surface zeta-potential (ζ) value of about +20 mV The conjugated PEG molecules suppressed any KALA-mediated inter-particle aggregation, and thereby improved the transfection efficiency. Consequently, the transfection efficiency of the KALA/PEG-g-PEI/DNA complexes was obtained by utilizing both the fusogenic activity of KALA and the steric repulsion effect of PEG.

Original language	English
Pages (from-to)	269-273
Number of pages	5
Journal	Biotechnology and Bioprocess Engineering
Volume	6
Issue number	4
DOIs	https://doi.org/10.1007/BF02931988
State	Published - 2001
Externally published	Yes

Funding

Acknowledgements This work was supported by the Ministry of Health and Welfare (HMP-99-B-02-0002), Republic of Korea.

Funders	Funder number
Ministry of Health and Welfare	HMP-99-B-02-0002

Keywords

A DNA delivery system
KALA
Polyethylene glycol (PEG)
Polyethylenimine (PEI)

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10.1007/BF02931988

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title = "Enhancing transfection efficiency using polyethylene glycol grafted polyethylenimine and fusogenic peptide",

abstract = "This study presents a new formulation method for improving DNA transfection efficiency using a fusogenic peptide and polyethylene glycol grafted polyethylenimine. Succinimidyl succinate polyethylene glycol (PEG-SSA) was conjugated with polyethylenimine (PEI). PEI is well known for a good endosomal escaping and DNA condensing agent. The positively charged synthetic fusogenic peptide, KALA, was coated on the negatively charged PEG-g-PEI/DNA and PEI/DNA complexes. The KALA/PEI/DNA complexes exhibited aggregation behavior at higher KALA coating amounts with an effective diameter of around 1,000 nm. However, the KALA/PEG-g-PEI/DNA complexes were 100-300 nm in size with a surface zeta-potential (ζ) value of about +20 mV The conjugated PEG molecules suppressed any KALA-mediated inter-particle aggregation, and thereby improved the transfection efficiency. Consequently, the transfection efficiency of the KALA/PEG-g-PEI/DNA complexes was obtained by utilizing both the fusogenic activity of KALA and the steric repulsion effect of PEG.",

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year = "2001",

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T1 - Enhancing transfection efficiency using polyethylene glycol grafted polyethylenimine and fusogenic peptide

AU - Lee, Haeshin

AU - Jeong, Ji Hoon

AU - Lee, Je Hoon

AU - Park, Tae Gwan

PY - 2001

Y1 - 2001

N2 - This study presents a new formulation method for improving DNA transfection efficiency using a fusogenic peptide and polyethylene glycol grafted polyethylenimine. Succinimidyl succinate polyethylene glycol (PEG-SSA) was conjugated with polyethylenimine (PEI). PEI is well known for a good endosomal escaping and DNA condensing agent. The positively charged synthetic fusogenic peptide, KALA, was coated on the negatively charged PEG-g-PEI/DNA and PEI/DNA complexes. The KALA/PEI/DNA complexes exhibited aggregation behavior at higher KALA coating amounts with an effective diameter of around 1,000 nm. However, the KALA/PEG-g-PEI/DNA complexes were 100-300 nm in size with a surface zeta-potential (ζ) value of about +20 mV The conjugated PEG molecules suppressed any KALA-mediated inter-particle aggregation, and thereby improved the transfection efficiency. Consequently, the transfection efficiency of the KALA/PEG-g-PEI/DNA complexes was obtained by utilizing both the fusogenic activity of KALA and the steric repulsion effect of PEG.

AB - This study presents a new formulation method for improving DNA transfection efficiency using a fusogenic peptide and polyethylene glycol grafted polyethylenimine. Succinimidyl succinate polyethylene glycol (PEG-SSA) was conjugated with polyethylenimine (PEI). PEI is well known for a good endosomal escaping and DNA condensing agent. The positively charged synthetic fusogenic peptide, KALA, was coated on the negatively charged PEG-g-PEI/DNA and PEI/DNA complexes. The KALA/PEI/DNA complexes exhibited aggregation behavior at higher KALA coating amounts with an effective diameter of around 1,000 nm. However, the KALA/PEG-g-PEI/DNA complexes were 100-300 nm in size with a surface zeta-potential (ζ) value of about +20 mV The conjugated PEG molecules suppressed any KALA-mediated inter-particle aggregation, and thereby improved the transfection efficiency. Consequently, the transfection efficiency of the KALA/PEG-g-PEI/DNA complexes was obtained by utilizing both the fusogenic activity of KALA and the steric repulsion effect of PEG.

KW - A DNA delivery system

KW - KALA

KW - Polyethylene glycol (PEG)

KW - Polyethylenimine (PEI)

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DO - 10.1007/BF02931988

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SN - 1226-8372

VL - 6

SP - 269

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JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 4

ER -

Enhancing transfection efficiency using polyethylene glycol grafted polyethylenimine and fusogenic peptide

Abstract

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