Abstract
Pseudomonas putida F-1 (ATCC 700007) was used as a model organism in stirred tank reactors to study conversion enhancement of poorly soluble substrates by organic cosolvents. After a literature study, silicone oil was used as a solvent system to enhance the mass transfer rate. To study the benefits of the organic solvent addition, batch experiments were conducted in two side-by-side fermentation vessels (experimental and control) at three different levels of silicone oil (10, 30, and 50%). Results showed that the presence of silicone oil resulted in a 100% increase in the toluene mass transfer compared to the control. Experiments in continuous stirred-tank reactors showed that improved conversion could be obtained at higher agitation rates.
| Original language | English |
|---|---|
| Pages (from-to) | 195-204 |
| Number of pages | 10 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume | 91-93 |
| DOIs | |
| State | Published - 2001 |
Funding
This work was supported by the Advanced Research and Technology Development Program of the Office of Fossil Energy. Oak Ridge National Laboratory is managed and operated for the U.S. Department of Energy by UT-Battelle, LLC under contract DE-AC05-00OR22725. This article was authored by a contractor of the U.S. Government under contract DE-AC05-00OR22725. Accordingly, the U.S. Government retains a nonexclusive royalty-free license to publish or reproduce the published form of the contribution, or allow others to do so, for U.S. Government purposes.
Keywords
- Organic solvents
- Pseudomonas putida F-1
- Silicone oil
- Toluene