Abstract
Nontoxic, biocompatible nanodiamonds (ND) have recently been implemented in rational, systematic design of optimal therapeutic use in nanomedicines. However, hydrophilicity of the ND surface strongly influences structure and dynamics of biomolecules that restrict in situ applications of ND. Therefore, fundamental understanding of the impact of hydrophilic ND surface on biomolecules at the molecular level is essential. For tRNA, we observe an enhancement of dynamical behavior in the presence of ND contrary to generally observed slow motion at strongly interacting interfaces. We took advantage of neutron scattering experiments and computer simulations to demonstrate this atypical faster dynamics of tRNA on ND surface. The strong attractive interactions between ND, tRNA, and water give rise to unlike dynamical behavior and structural changes of tRNA in front of ND compared to without ND. Our new findings may provide new design principles for safer, improved drug delivery platforms.
Original language | English |
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Pages (from-to) | 10059-10068 |
Number of pages | 10 |
Journal | Journal of Physical Chemistry B |
Volume | 120 |
Issue number | 38 |
DOIs | |
State | Published - Sep 29 2016 |
Funding
This work was supported by Wayne State University and the U.S. Department of Energy (DoE), Office of Basic Energy Sciences, Materials Science and Engineering Division. Research by M.G. is supported by the Center for Accelerated Materials Modeling (CAMM) funded by the U.S. DoE, BES, MSED. B.G.S. and P.G. acknowledge work performed at the Center for Nanophase Materials Sciences, a DoE Office of Science User Facility. This research used resources of the Oak Ridge Leadership Computing Facility at the Oak Ridge National Laboratory, which is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC05-00OR22725 and also National Energy Research Scientific Computing Center, a DoE Office of Science User Facility supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. H.O’N. acknowledges support from the Center for Structural Molecular Biology funded by the U.S. DOE, Office of Science, Office of Biological and Environmental Research (OBER) Project ERKP291. Neutron scattering measurements at ORNL’s Spallation Neutron Source were supported by the Scientific User Facilities Division, Office of Basic Energy Sciences, U.S. Department of Energy. The authors gratefully acknowledge the generous help from Vadym Mochalin and Yury Gogotsi for providing the nanodiamond samples.