Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum

Hannah O. Akinosho; Chang Geun Yoo; Alexandru Dumitrache; Jace Natzke; Wellington Muchero; Steven D. Brown; Arthur J. Ragauskas

doi:10.1021/acssuschemeng.7b01203

Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum

Hannah O. Akinosho, Chang Geun Yoo, Alexandru Dumitrache, Jace Natzke, Wellington Muchero, Steven D. Brown, Arthur J. Ragauskas

Plant Systems Biology

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

During consolidated bioprocessing (CBP), Clostridium thermocellum hydrolyzes several plant cell wall components. Cellulose hydrolysis, specifically, liberates sugars for fermentation, which generates ethanol, acetate, hydrogen, and other products. While several studies indicate that C. thermocellum hydrolyzes carbohydrates in biomass, the structural changes to lignin during CBP remain unclear. In this study, the whole plant cell walls of untreated and C. thermocellum-treated Populus trichocarpa were characterized using NMR and FTIR. The results suggest that C. thermocellum reduces the β-O-4 linkage content and increases the lignin S/G ratio. This investigation indicates that C. thermocellum not only modifies lignin in order to access cellulose but also leaves behind a suitable lignin substrate for value-added applications in the cellulosic ethanol production scheme.

Original language	English
Pages (from-to)	7486-7491
Number of pages	6
Journal	ACS Sustainable Chemistry and Engineering
Volume	5
Issue number	9
DOIs	https://doi.org/10.1021/acssuschemeng.7b01203
State	Published - Sep 5 2017

Funding

We greatly appreciate the Renewable BioProducts Institute at Georgia Institute of Technology for their financial support of H.O.A. through the RBI Fellowship for graduate studies. Funding provided by The BioEnergy Science Center a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. This manuscript has been authored by UT-Battelle, LLC under Contract No. DE-AC05-00OR22725 with the U.S. Department of Energy.

Funders	Funder number
DOE Office of Science
Office of Biological and Environmental Research
Georgia Institute of Technology
Center for Bioenergy Innovation

Keywords

Clostridium thermocellum
Consolidated bioprocessing
FTIR
Lignin characterization
NMR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acssuschemeng.7b01203

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title = "Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum",

abstract = "During consolidated bioprocessing (CBP), Clostridium thermocellum hydrolyzes several plant cell wall components. Cellulose hydrolysis, specifically, liberates sugars for fermentation, which generates ethanol, acetate, hydrogen, and other products. While several studies indicate that C. thermocellum hydrolyzes carbohydrates in biomass, the structural changes to lignin during CBP remain unclear. In this study, the whole plant cell walls of untreated and C. thermocellum-treated Populus trichocarpa were characterized using NMR and FTIR. The results suggest that C. thermocellum reduces the β-O-4 linkage content and increases the lignin S/G ratio. This investigation indicates that C. thermocellum not only modifies lignin in order to access cellulose but also leaves behind a suitable lignin substrate for value-added applications in the cellulosic ethanol production scheme.",

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AU - Akinosho, Hannah O.

AU - Yoo, Chang Geun

AU - Dumitrache, Alexandru

AU - Natzke, Jace

AU - Muchero, Wellington

AU - Brown, Steven D.

AU - Ragauskas, Arthur J.

PY - 2017/9/5

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N2 - During consolidated bioprocessing (CBP), Clostridium thermocellum hydrolyzes several plant cell wall components. Cellulose hydrolysis, specifically, liberates sugars for fermentation, which generates ethanol, acetate, hydrogen, and other products. While several studies indicate that C. thermocellum hydrolyzes carbohydrates in biomass, the structural changes to lignin during CBP remain unclear. In this study, the whole plant cell walls of untreated and C. thermocellum-treated Populus trichocarpa were characterized using NMR and FTIR. The results suggest that C. thermocellum reduces the β-O-4 linkage content and increases the lignin S/G ratio. This investigation indicates that C. thermocellum not only modifies lignin in order to access cellulose but also leaves behind a suitable lignin substrate for value-added applications in the cellulosic ethanol production scheme.

AB - During consolidated bioprocessing (CBP), Clostridium thermocellum hydrolyzes several plant cell wall components. Cellulose hydrolysis, specifically, liberates sugars for fermentation, which generates ethanol, acetate, hydrogen, and other products. While several studies indicate that C. thermocellum hydrolyzes carbohydrates in biomass, the structural changes to lignin during CBP remain unclear. In this study, the whole plant cell walls of untreated and C. thermocellum-treated Populus trichocarpa were characterized using NMR and FTIR. The results suggest that C. thermocellum reduces the β-O-4 linkage content and increases the lignin S/G ratio. This investigation indicates that C. thermocellum not only modifies lignin in order to access cellulose but also leaves behind a suitable lignin substrate for value-added applications in the cellulosic ethanol production scheme.

KW - Clostridium thermocellum

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KW - Lignin characterization

KW - NMR

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Elucidating the Structural Changes to Populus Lignin during Consolidated Bioprocessing with Clostridium thermocellum

Abstract

Funding

Keywords

UN SDGs

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