Electromechanical imaging of biomaterials by scanning probe microscopy

B. J. Rodriguez; S. V. Kalinin; J. Shin; S. Jesse; V. Grichko; T. Thundat; A. P. Baddorf; A. Gruverman

doi:10.1016/j.jsb.2005.10.008

Electromechanical imaging of biomaterials by scanning probe microscopy

B. J. Rodriguez, S. V. Kalinin, J. Shin, S. Jesse, V. Grichko, T. Thundat, A. P. Baddorf, A. Gruverman

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

The majority of calcified and connective tissues possess complex hierarchical structure spanning the length scales from nanometers to millimeters. Understanding the biological functionality of these materials requires reliable methods for structural imaging on the nanoscale. Here, we demonstrate an approach for electromechanical imaging of the structure of biological samples on the length scales from tens of microns to nanometers using piezoresponse force microscopy (PFM), which utilizes the intrinsic piezoelectricity of biopolymers such as proteins and polysaccharides as the basis for high-resolution imaging. Nanostructural imaging of a variety of protein-based materials, including tooth, antler, and cartilage, is demonstrated. Visualization of protein fibrils with sub-10 nm spatial resolution in a human tooth is achieved. Given the near-ubiquitous presence of piezoelectricity in biological systems, PFM is suggested as a versatile tool for micro- and nanostructural imaging in both connective and calcified tissues.

Original language	English
Pages (from-to)	151-159
Number of pages	9
Journal	Journal of Structural Biology
Volume	153
Issue number	2
DOIs	https://doi.org/10.1016/j.jsb.2005.10.008
State	Published - Feb 2006

Funding

Research performed in part as a Eugene P. Wigner Fellow and staff member at the Oak Ridge National Laboratory, managed by UT-Battelle, LLC, for the U.S. Department of Energy under Contract No. DE-AC05-00OR22725 (S.V.K.). Support from ORNL SEED funding is acknowledged (S.V.K. and T.T.). A.G. acknowledges financial support of the National Science Foundation (Grant No. DMR02-35632). The authors acknowledge M. Yamauchi (UNC) and E. Loboa (NCSU) for collagen and cartilage samples, respectively.

Funders	Funder number
National Science Foundation
U.S. Department of Energy

Keywords

Calcified tissues
Connective tissues
Nanoscale
Piezoelectricity
Piezoresponse force microscopy
Scanning probe microscopy

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10.1016/j.jsb.2005.10.008

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title = "Electromechanical imaging of biomaterials by scanning probe microscopy",

abstract = "The majority of calcified and connective tissues possess complex hierarchical structure spanning the length scales from nanometers to millimeters. Understanding the biological functionality of these materials requires reliable methods for structural imaging on the nanoscale. Here, we demonstrate an approach for electromechanical imaging of the structure of biological samples on the length scales from tens of microns to nanometers using piezoresponse force microscopy (PFM), which utilizes the intrinsic piezoelectricity of biopolymers such as proteins and polysaccharides as the basis for high-resolution imaging. Nanostructural imaging of a variety of protein-based materials, including tooth, antler, and cartilage, is demonstrated. Visualization of protein fibrils with sub-10 nm spatial resolution in a human tooth is achieved. Given the near-ubiquitous presence of piezoelectricity in biological systems, PFM is suggested as a versatile tool for micro- and nanostructural imaging in both connective and calcified tissues.",

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AU - Shin, J.

AU - Jesse, S.

AU - Grichko, V.

AU - Thundat, T.

AU - Baddorf, A. P.

AU - Gruverman, A.

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KW - Calcified tissues

KW - Connective tissues

KW - Nanoscale

KW - Piezoelectricity

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Electromechanical imaging of biomaterials by scanning probe microscopy

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