TY - JOUR
T1 - Electrocatalytic tuning of biosensing response through electrostatic or hydrophobic enzyme-graphene oxide interactions
AU - Baptista-Pires, Luis
AU - Pérez-López, Briza
AU - Mayorga-Martinez, Carmen C.
AU - Morales-Narváez, Eden
AU - Domingo, Neus
AU - Esplandiu, Maria Jose
AU - Alzina, Francesc
AU - Torres, Clivia M.Sotomayor
AU - Merkoçi, Arben
PY - 2014/11/15
Y1 - 2014/11/15
N2 - The effect of graphene oxidative grades upon the conductivity and hydrophobicity and consequently the influence on an enzymatic biosensing response is presented. The electrochemical responses of reduced graphene oxide (rGO) have been compared with the responses obtained from the oxide form (oGO) and their performances have been accordingly discussed with various evidences obtained by optical techniques. We used tyrosinase enzyme as a proof of concept receptor with interest for phenolic compounds detection through its direct adsorption onto a screen-printed carbon electrode previously modified with oGO or rGO with a carbon-oxygen ratio of 1.07 or 1.53 respectively. Different levels of oGO directly affect the (bio)conjugation properties of the biosensor due to changes at enzyme/graphene oxide interface coming from the various electrostatic or hydrophobic interactions with biomolecules. The developed biosensor was capable of reaching a limit of detection of 0.01. nM catechol. This tuning capability of the biosensor response can be of interest for building several other biosensors, including immunosensors and DNA sensors for various applications.
AB - The effect of graphene oxidative grades upon the conductivity and hydrophobicity and consequently the influence on an enzymatic biosensing response is presented. The electrochemical responses of reduced graphene oxide (rGO) have been compared with the responses obtained from the oxide form (oGO) and their performances have been accordingly discussed with various evidences obtained by optical techniques. We used tyrosinase enzyme as a proof of concept receptor with interest for phenolic compounds detection through its direct adsorption onto a screen-printed carbon electrode previously modified with oGO or rGO with a carbon-oxygen ratio of 1.07 or 1.53 respectively. Different levels of oGO directly affect the (bio)conjugation properties of the biosensor due to changes at enzyme/graphene oxide interface coming from the various electrostatic or hydrophobic interactions with biomolecules. The developed biosensor was capable of reaching a limit of detection of 0.01. nM catechol. This tuning capability of the biosensor response can be of interest for building several other biosensors, including immunosensors and DNA sensors for various applications.
KW - Electrocatalytic tuning
KW - Electrostatic interactions
KW - Hydrophobic interactions
KW - Oxidized graphene oxide
KW - Reduced graphene oxide
UR - http://www.scopus.com/inward/record.url?scp=84903388371&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2014.05.028
DO - 10.1016/j.bios.2014.05.028
M3 - Article
C2 - 24976046
AN - SCOPUS:84903388371
SN - 0956-5663
VL - 61
SP - 655
EP - 662
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -