Electric field induced bacterial flocculation of enteroaggregative Escherichia coli 042

Aloke Kumar, Ninell P. Mortensen, Partha P. Mukherjee, Scott T. Retterer, Mitchel J. Doktycz

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Abstract

A response of the aggregation dynamics of enteroaggregative Escherichia coli under low magnitude steady and oscillating electric fields is presented. The presence of uniform electric fields hampered microbial adhesion and biofilm formation on a transverse glass surface, but instead promoted the formation of flocs. Extremely heterogenous distribution of live and dead cells was observed among the flocs. Moreover, floc formation was largely observed to be independent of the frequency of alternating electric fields.

Original languageEnglish
Article number253701
JournalApplied Physics Letters
Volume98
Issue number25
DOIs
StatePublished - Jun 20 2011

Funding

The authors would like to thank Dr. James P. Nataro, University of Virginia School of Medicine for the strain EAEC 042 and 042aap. A.K. performed the work as a Eugene P. Wigner Fellow and staff member at the Oak Ridge National Laboratory (ORNL). A portion of this research was conducted at the Center for Nanophase Materials Sciences, which is sponsored at ORNL by the Scientific User Facilities Division, U.S. Department of Energy (U.S. DOE). The authors acknowledge research support from the U.S. DOE Office of Biological and Environmental Sciences. ORNL is managed by UT-Battelle, LLC, for the U.S. DOE under Contract No. DEAC05-00OR22725.

FundersFunder number
U.S. DOE Office of Biological and Environmental Sciences
U.S. Department of EnergyDEAC05-00OR22725
Oak Ridge National Laboratory

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