Elastic and conformational softness of a globular protein

Liang Hong, Dennis C. Glass, Jonathan D. Nickels, Stefania Perticaroli, Zheng Yi, Tyagi Madhusudan, Hugh O'Neill, Qiu Zhang, Alexei P. Sokolov, Jeremy C. Smith

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Flexibility, or softness, is crucial for protein function and consists of a conformational component, involving jumps between potential wells, and an elastic component, involving fluctuations within the wells. Combining molecular dynamics simulation with incoherent neutron scattering and light scattering measurements on green fluorescent protein, we reveal a relationship between the intrawell fluctuations and elastic moduli of the protein. This finding leads to a simple means of experimentally separating the conformational from the elastic atomic displacements.

Original languageEnglish
Article number028104
JournalPhysical Review Letters
Volume110
Issue number2
DOIs
StatePublished - Jan 10 2013

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