Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa

Hannah Akinosho; Alexandru Dumitrache; Jace Natzke; Wellington Muchero; Sara S. Jawdy; Gerald A. Tuskan; Steven D. Brown; Arthur J. Ragauskas

doi:10.1021/acssuschemeng.7b00449

Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa

Hannah Akinosho, Alexandru Dumitrache, Jace Natzke, Wellington Muchero, Sara S. Jawdy, Gerald A. Tuskan, Steven D. Brown, Arthur J. Ragauskas

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The bacterium Clostridium thermocellum offers a distinct and integrated approach to ethanol production through consolidated bioprocessing (CBP). The Simons' stain technique, which assays the accessibility of lignocellulosic biomass, has been traditionally applied to fungal cellulase systems; however, its application to CBP has not been fully explored. For this reason, the structural properties of eight Populus trichocarpa with either high or low biomass densities were compared to determine bioconversion differences during separate hydrolysis and fermentation (SHF) and CBP with C. thermocellum. Simons' staining generally identifies low density poplar as more accessible than high density poplar. Additionally, low density P. trichocarpa generally contained less Klason lignin than high density poplar. SHF and CBP treatments consistently identified BESC-7 (high density, low accessibility, low surface roughness) as a low ethanol yielding biomass and GW-9914 (low density, high accessibility, high surface roughness) as a high ethanol yielding biomass. Upon further investigation, BESC-7 also contained a high Klason lignin content (∼25%), while GW-9914 had a low lignin content (∼20%). Cellulose degree of polymerization (DP) measurements exhibited a weak linear correlation with accessibility (r² = 0.17). Therefore, the ethanol yields were correlated with accessibility and lignin content extremes but not cellulose DP.

Original language	English
Pages (from-to)	5075-5081
Number of pages	7
Journal	ACS Sustainable Chemistry and Engineering
Volume	5
Issue number	6
DOIs	https://doi.org/10.1021/acssuschemeng.7b00449
State	Published - Jun 5 2017

Funding

We would like to acknowledge the Renewable BioProducts Institute at Georgia Institute of Technology for their financial support of H.A. through the RBI fellowship. We would also like to acknowledge the Center for Nanophase Materials Sciences (CNMS), which is a DOE Office of Science User Facility for the use of the surface profilometer. We appreciate Bernadeta Srijanto, Nickolay Lavrik, and Dayrl Briggs for their support of this research through CNMS. The research was conducted under the BioEnergy Science Center (BESC) at Oak Ridge National Laboratory. BESC is a U.S. Department of Energy (DOE) Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. Oak Ridge National Laboratory is managed by UTBattelle, LLC, who authored the manuscript under Contract DE-AC05-00OR22725 with the U.S. Department of Energy.

Funders	Funder number
BioEnergy Science Center
Center for Nanophase Materials Sciences
U.S. Department of Energy
Office of Science
Biological and Environmental Research	DE-AC05-00OR22725
Oak Ridge National Laboratory
Georgia Institute of Technology

Keywords

Clostridium thermocellum
Klason lignin
Simons' stain
consolidated bioprocessing
ethanol
wood density

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10.1021/acssuschemeng.7b00449

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title = "Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa",

abstract = "The bacterium Clostridium thermocellum offers a distinct and integrated approach to ethanol production through consolidated bioprocessing (CBP). The Simons' stain technique, which assays the accessibility of lignocellulosic biomass, has been traditionally applied to fungal cellulase systems; however, its application to CBP has not been fully explored. For this reason, the structural properties of eight Populus trichocarpa with either high or low biomass densities were compared to determine bioconversion differences during separate hydrolysis and fermentation (SHF) and CBP with C. thermocellum. Simons' staining generally identifies low density poplar as more accessible than high density poplar. Additionally, low density P. trichocarpa generally contained less Klason lignin than high density poplar. SHF and CBP treatments consistently identified BESC-7 (high density, low accessibility, low surface roughness) as a low ethanol yielding biomass and GW-9914 (low density, high accessibility, high surface roughness) as a high ethanol yielding biomass. Upon further investigation, BESC-7 also contained a high Klason lignin content (∼25%), while GW-9914 had a low lignin content (∼20%). Cellulose degree of polymerization (DP) measurements exhibited a weak linear correlation with accessibility (r2 = 0.17). Therefore, the ethanol yields were correlated with accessibility and lignin content extremes but not cellulose DP.",

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AU - Muchero, Wellington

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Effects of Biomass Accessibility and Klason Lignin Contents during Consolidated Bioprocessing in Populus trichocarpa

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