Effect of cholesterol on the lateral nanoscale dynamics of fluid membranes

Clare L. Armstrong; Matthew A. Barrett; Arno Hiess; Tim Salditt; John Katsaras; An Chang Shi; Maikel C. Rheinstädter

doi:10.1007/s00249-012-0826-4

Effect of cholesterol on the lateral nanoscale dynamics of fluid membranes

Clare L. Armstrong
, Matthew A. Barrett
, Arno Hiess
, Tim Salditt
, John Katsaras
, An Chang Shi
, Maikel C. Rheinstädter

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

Inelastic neutron scattering was used to study the effect of 5 and 40 mol% cholesterol on the lateral nanoscale dynamics of phospholipid membranes. By measuring the excitation spectrum at several lateral q|| values (up to q|| = 3 A -1), complete dispersion curves were determined of gel, fluid and liquid-ordered phase bilayers. The inclusion of cholesterol had a distinct effect on the collective dynamics of the bilayer's hydrocarbon chains; specifically, we observed a pronounced stiffening of the membranes on the nanometer length scale in both gel and fluid bilayers, even though they were experiencing a higher degree of molecular disorder. Also, for the first time we determined the nanoscale dynamics in the highcholesterol liquid-ordered phase of bilayers containing cholesterol. Namely, this phase appears to be ''softer'' than fluid bilayers, but better ordered than bilayers in the gel phase.

Original language	English
Pages (from-to)	901-913
Number of pages	13
Journal	European Biophysics Journal
Volume	41
Issue number	10
DOIs	https://doi.org/10.1007/s00249-012-0826-4
State	Published - Oct 2012

Funding

Acknowledgments This research was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC), the National Research Council Canada (NRC), the Canada Foundation for Innovation (CFI) and the Ontario Ministry of Economic Development and Innovation. John Katsaras is supported by Oak Ridge National Laboratory’s (ORNL) Program Development (PD) and Laboratory Directed Research and Development (LDRD) programs.

Keywords

Cholesterol
Dispersion relation
Inelastic neutron scattering
Lateral membrane dynamics
Lipid membrane
Liquidordered phase
Nanoscale dynamics

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10.1007/s00249-012-0826-4

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title = "Effect of cholesterol on the lateral nanoscale dynamics of fluid membranes",

abstract = "Inelastic neutron scattering was used to study the effect of 5 and 40 mol\% cholesterol on the lateral nanoscale dynamics of phospholipid membranes. By measuring the excitation spectrum at several lateral q|| values (up to q|| = 3 A -1), complete dispersion curves were determined of gel, fluid and liquid-ordered phase bilayers. The inclusion of cholesterol had a distinct effect on the collective dynamics of the bilayer's hydrocarbon chains; specifically, we observed a pronounced stiffening of the membranes on the nanometer length scale in both gel and fluid bilayers, even though they were experiencing a higher degree of molecular disorder. Also, for the first time we determined the nanoscale dynamics in the highcholesterol liquid-ordered phase of bilayers containing cholesterol. Namely, this phase appears to be ''softer'' than fluid bilayers, but better ordered than bilayers in the gel phase.",

keywords = "Cholesterol, Dispersion relation, Inelastic neutron scattering, Lateral membrane dynamics, Lipid membrane, Liquidordered phase, Nanoscale dynamics",

author = "Armstrong, \{Clare L.\} and Barrett, \{Matthew A.\} and Arno Hiess and Tim Salditt and John Katsaras and Shi, \{An Chang\} and Rheinst{\"a}dter, \{Maikel C.\}",

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AU - Armstrong, Clare L.

AU - Barrett, Matthew A.

AU - Hiess, Arno

AU - Salditt, Tim

AU - Katsaras, John

AU - Shi, An Chang

AU - Rheinstädter, Maikel C.

PY - 2012/10

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N2 - Inelastic neutron scattering was used to study the effect of 5 and 40 mol% cholesterol on the lateral nanoscale dynamics of phospholipid membranes. By measuring the excitation spectrum at several lateral q|| values (up to q|| = 3 A -1), complete dispersion curves were determined of gel, fluid and liquid-ordered phase bilayers. The inclusion of cholesterol had a distinct effect on the collective dynamics of the bilayer's hydrocarbon chains; specifically, we observed a pronounced stiffening of the membranes on the nanometer length scale in both gel and fluid bilayers, even though they were experiencing a higher degree of molecular disorder. Also, for the first time we determined the nanoscale dynamics in the highcholesterol liquid-ordered phase of bilayers containing cholesterol. Namely, this phase appears to be ''softer'' than fluid bilayers, but better ordered than bilayers in the gel phase.

AB - Inelastic neutron scattering was used to study the effect of 5 and 40 mol% cholesterol on the lateral nanoscale dynamics of phospholipid membranes. By measuring the excitation spectrum at several lateral q|| values (up to q|| = 3 A -1), complete dispersion curves were determined of gel, fluid and liquid-ordered phase bilayers. The inclusion of cholesterol had a distinct effect on the collective dynamics of the bilayer's hydrocarbon chains; specifically, we observed a pronounced stiffening of the membranes on the nanometer length scale in both gel and fluid bilayers, even though they were experiencing a higher degree of molecular disorder. Also, for the first time we determined the nanoscale dynamics in the highcholesterol liquid-ordered phase of bilayers containing cholesterol. Namely, this phase appears to be ''softer'' than fluid bilayers, but better ordered than bilayers in the gel phase.

KW - Cholesterol

KW - Dispersion relation

KW - Inelastic neutron scattering

KW - Lateral membrane dynamics

KW - Lipid membrane

KW - Liquidordered phase

KW - Nanoscale dynamics

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Effect of cholesterol on the lateral nanoscale dynamics of fluid membranes

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Keywords

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