Effect of catalase on hydrogen peroxide penetration into Pseudomonas aeruginosa biofilms

Philip S. Stewart; Frank Roe; Joanna Rayner; James G. Elkins; Zbigniew Lewandowski; Urs A. Ochsner; Daniel J. Hassett

doi:10.1128/AEM.66.2.836-838.2000

Effect of catalase on hydrogen peroxide penetration into Pseudomonas aeruginosa biofilms

Philip S. Stewart, Frank Roe, Joanna Rayner, James G. Elkins, Zbigniew Lewandowski, Urs A. Ochsner, Daniel J. Hassett

Research output: Contribution to journal › Article › peer-review

149 Scopus citations

Abstract

The penetration of hydrogen peroxide into biofilms formed by wild-type and catalase-deficient Pseudomonas aeruginosa strains was measured using microelectrodes. A flowing stream of hydrogen peroxide (50 mM, 1 h) was unable to penetrate or kill wild-type biofilms but did penetrate and partially kill biofilms formed by an isogenic strain in which the katA gene was knocked out. Catalase protects aggregated bacteria by preventing full penetration of hydrogen peroxide into the biofilm.

Original language	English
Pages (from-to)	836-838
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	66
Issue number	2
DOIs	https://doi.org/10.1128/AEM.66.2.836-838.2000
State	Published - Feb 2000
Externally published	Yes

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10.1128/AEM.66.2.836-838.2000

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abstract = "The penetration of hydrogen peroxide into biofilms formed by wild-type and catalase-deficient Pseudomonas aeruginosa strains was measured using microelectrodes. A flowing stream of hydrogen peroxide (50 mM, 1 h) was unable to penetrate or kill wild-type biofilms but did penetrate and partially kill biofilms formed by an isogenic strain in which the katA gene was knocked out. Catalase protects aggregated bacteria by preventing full penetration of hydrogen peroxide into the biofilm.",

author = "Stewart, {Philip S.} and Frank Roe and Joanna Rayner and Elkins, {James G.} and Zbigniew Lewandowski and Ochsner, {Urs A.} and Hassett, {Daniel J.}",

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AU - Stewart, Philip S.

AU - Roe, Frank

AU - Rayner, Joanna

AU - Elkins, James G.

AU - Lewandowski, Zbigniew

AU - Ochsner, Urs A.

AU - Hassett, Daniel J.

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AB - The penetration of hydrogen peroxide into biofilms formed by wild-type and catalase-deficient Pseudomonas aeruginosa strains was measured using microelectrodes. A flowing stream of hydrogen peroxide (50 mM, 1 h) was unable to penetrate or kill wild-type biofilms but did penetrate and partially kill biofilms formed by an isogenic strain in which the katA gene was knocked out. Catalase protects aggregated bacteria by preventing full penetration of hydrogen peroxide into the biofilm.

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Effect of catalase on hydrogen peroxide penetration into Pseudomonas aeruginosa biofilms

Abstract

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