Dissolution of biomacromolecules in organic solvents by nano-complexing with poly(ethylene glycol)

Hyejung Mok; Ho Jeong Kim; Tae Gwan Park

doi:10.1016/j.ijpharm.2008.01.033

Dissolution of biomacromolecules in organic solvents by nano-complexing with poly(ethylene glycol)

Hyejung Mok, Ho Jeong Kim, Tae Gwan Park

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Various biomacromolecules (BMs) such as proteins, DNA, and carbohydrates are extremely difficult to be dissolved in a single organic solvent phase for sustained release or targeted delivery formulation. In this study, three different BMs could be solubilized in selected organic solvents by forming poly(ethylene glycol) (PEG)-assisted nano-complexes while maintaining their structural integrity. Dynamic light scattering (DLS) and atomic force microscopy (AFM) analysis revealed that proteins, DNA, and carbohydrate polymers could be nano-complexed with PEG in various organic solvents. The diameter of nano-complexes decreased roughly from ∼600 nm to ∼100 nm with increasing weight ratio of PEG/BM. The present solubilization technique could be potentially applied for sustained release formulations of various therapeutic biological drugs.

Original language	English
Pages (from-to)	306-313
Number of pages	8
Journal	International Journal of Pharmaceutics
Volume	356
Issue number	1-2
DOIs	https://doi.org/10.1016/j.ijpharm.2008.01.033
State	Published - May 22 2008
Externally published	Yes

Funding

This study was supported by the grant from the National Research Laboratory project from the Ministry of Science and Technology, Republic of Korea.

Funders	Funder number
U.S. Naval Research Laboratory
Ministry of Science and Technology

Keywords

Biomacromolecules
Bovine serum albumin
DNA
Hyaluronic acid
Poly(ethylene glycol)
Solubilization in organic solvents

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10.1016/j.ijpharm.2008.01.033

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title = "Dissolution of biomacromolecules in organic solvents by nano-complexing with poly(ethylene glycol)",

abstract = "Various biomacromolecules (BMs) such as proteins, DNA, and carbohydrates are extremely difficult to be dissolved in a single organic solvent phase for sustained release or targeted delivery formulation. In this study, three different BMs could be solubilized in selected organic solvents by forming poly(ethylene glycol) (PEG)-assisted nano-complexes while maintaining their structural integrity. Dynamic light scattering (DLS) and atomic force microscopy (AFM) analysis revealed that proteins, DNA, and carbohydrate polymers could be nano-complexed with PEG in various organic solvents. The diameter of nano-complexes decreased roughly from ∼600 nm to ∼100 nm with increasing weight ratio of PEG/BM. The present solubilization technique could be potentially applied for sustained release formulations of various therapeutic biological drugs.",

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AU - Mok, Hyejung

AU - Kim, Ho Jeong

AU - Park, Tae Gwan

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Y1 - 2008/5/22

N2 - Various biomacromolecules (BMs) such as proteins, DNA, and carbohydrates are extremely difficult to be dissolved in a single organic solvent phase for sustained release or targeted delivery formulation. In this study, three different BMs could be solubilized in selected organic solvents by forming poly(ethylene glycol) (PEG)-assisted nano-complexes while maintaining their structural integrity. Dynamic light scattering (DLS) and atomic force microscopy (AFM) analysis revealed that proteins, DNA, and carbohydrate polymers could be nano-complexed with PEG in various organic solvents. The diameter of nano-complexes decreased roughly from ∼600 nm to ∼100 nm with increasing weight ratio of PEG/BM. The present solubilization technique could be potentially applied for sustained release formulations of various therapeutic biological drugs.

AB - Various biomacromolecules (BMs) such as proteins, DNA, and carbohydrates are extremely difficult to be dissolved in a single organic solvent phase for sustained release or targeted delivery formulation. In this study, three different BMs could be solubilized in selected organic solvents by forming poly(ethylene glycol) (PEG)-assisted nano-complexes while maintaining their structural integrity. Dynamic light scattering (DLS) and atomic force microscopy (AFM) analysis revealed that proteins, DNA, and carbohydrate polymers could be nano-complexed with PEG in various organic solvents. The diameter of nano-complexes decreased roughly from ∼600 nm to ∼100 nm with increasing weight ratio of PEG/BM. The present solubilization technique could be potentially applied for sustained release formulations of various therapeutic biological drugs.

KW - Biomacromolecules

KW - Bovine serum albumin

KW - DNA

KW - Hyaluronic acid

KW - Poly(ethylene glycol)

KW - Solubilization in organic solvents

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Dissolution of biomacromolecules in organic solvents by nano-complexing with poly(ethylene glycol)

Abstract

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Keywords

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