Differences in S/G ratio in natural poplar variants do not predict catalytic depolymerization monomer yields

Eric M. Anderson; Michael L. Stone; Rui Katahira; Michelle Reed; Wellington Muchero; Kelsey J. Ramirez; Gregg T. Beckham; Yuriy Román-Leshkov

doi:10.1038/s41467-019-09986-1

Differences in S/G ratio in natural poplar variants do not predict catalytic depolymerization monomer yields

Eric M. Anderson, Michael L. Stone, Rui Katahira, Michelle Reed, Wellington Muchero, Kelsey J. Ramirez, Gregg T. Beckham, Yuriy Román-Leshkov

Plant Systems Biology

Research output: Contribution to journal › Article › peer-review

146 Scopus citations

Abstract

The ratio of syringyl (S) and guaiacyl (G) units in lignin has been regarded as a major factor in determining the maximum monomer yield from lignin depolymerization. This limit arises from the notion that G units are prone to C-C bond formation during lignin biosynthesis, resulting in less ether linkages that generate monomers. This study uses reductive catalytic fractionation (RCF) in flow-through reactors as an analytical tool to depolymerize lignin in poplar with naturally varying S/G ratios, and directly challenges the common conception that the S/G ratio predicts monomer yields. Rather, this work suggests that the plant controls C-O and C-C bond content by regulating monomer transport during lignin biosynthesis. Overall, our results indicate that additional factors beyond the monomeric composition of native lignin are important in developing a fundamental understanding of lignin biosynthesis.

Original language	English
Article number	2033
Journal	Nature Communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-09986-1
State	Published - Dec 1 2019

Funding

Funding for E.M.A., M.L.S., W.M., G.T.B., and Y.R.-L. was provided by The Center for Bioenergy Innovation a U.S. Department of Energy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. Additionally, R.K., M.R., K.R., and G.T.B. thank the US Department of Energy Bioenergy Technologies Office for funding under Contract DE-AC36-08GO28308 with the National Renewable Energy Laboratory.

Funders	Funder number
Center for Bioenergy Innovation a U.S. Department of Energy Research Center
DOE Office of Science
Office of Biological and Environmental Research
US Department of Energy BioEnergy Technologies Office	DE-AC36-08GO28308
National Renewable Energy Laboratory

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abstract = "The ratio of syringyl (S) and guaiacyl (G) units in lignin has been regarded as a major factor in determining the maximum monomer yield from lignin depolymerization. This limit arises from the notion that G units are prone to C-C bond formation during lignin biosynthesis, resulting in less ether linkages that generate monomers. This study uses reductive catalytic fractionation (RCF) in flow-through reactors as an analytical tool to depolymerize lignin in poplar with naturally varying S/G ratios, and directly challenges the common conception that the S/G ratio predicts monomer yields. Rather, this work suggests that the plant controls C-O and C-C bond content by regulating monomer transport during lignin biosynthesis. Overall, our results indicate that additional factors beyond the monomeric composition of native lignin are important in developing a fundamental understanding of lignin biosynthesis.",

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AU - Muchero, Wellington

AU - Ramirez, Kelsey J.

AU - Beckham, Gregg T.

AU - Román-Leshkov, Yuriy

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Differences in S/G ratio in natural poplar variants do not predict catalytic depolymerization monomer yields

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