Dielectric constant of flagellin proteins measured by scanning dielectric microscopy

Helena Lozano; Rene Fabregas; Núria Blanco-Cabra; Rubén Millán-Solsona; Eduard Torrents; Laura Fumagalli; Gabriel Gomila

doi:10.1039/c8nr06190d

Dielectric constant of flagellin proteins measured by scanning dielectric microscopy

Helena Lozano
, Rene Fabregas
, Núria Blanco-Cabra
, Rubén Millán-Solsona
, Eduard Torrents
, Laura Fumagalli
, Gabriel Gomila

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The dielectric constant of flagellin proteins in flagellar bacterial filaments ∼10-20 nm in diameter is measured using scanning dielectric microscopy. We obtained for two different bacterial species (Shewanella oneidensis MR-1 and Pseudomonas aeruginosa PAO1) similar relative dielectric constant values ϵ_So = 4.3 ± 0.6 and ϵ_Pa = 4.5 ± 0.7, respectively, despite their different structure and amino acid sequence. The present results show the applicability of scanning dielectric microscopy to nanoscale filamentous protein complexes and to general 3D macromolecular protein geometries, thus opening new avenues to study the relationship between the dielectric response and protein structure and function.

Original language	English
Pages (from-to)	19188-19194
Number of pages	7
Journal	Nanoscale
Volume	10
Issue number	40
DOIs	https://doi.org/10.1039/c8nr06190d
State	Published - Oct 28 2018
Externally published	Yes

Funding

This work has been partially supported by the Spanish Agencia Estatal de Investigación and EU FEDER through grants TEC2015-72751-EXP, TEC2016-79156-P and BIO2015-63557-P. G. G. acknowledges support from an ICREA Academia award from the Generalitat de Catalunya and H. L. for an FPI grant (BES-2015-074799) from the Agencia Estatal de Investigación/ Fondo Social Europeo. We also acknowledge support from Generalitat de Catalunya (2017 SGR1079 and CERCA Program).

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title = "Dielectric constant of flagellin proteins measured by scanning dielectric microscopy",

abstract = "The dielectric constant of flagellin proteins in flagellar bacterial filaments ∼10-20 nm in diameter is measured using scanning dielectric microscopy. We obtained for two different bacterial species (Shewanella oneidensis MR-1 and Pseudomonas aeruginosa PAO1) similar relative dielectric constant values ϵSo = 4.3 ± 0.6 and ϵPa = 4.5 ± 0.7, respectively, despite their different structure and amino acid sequence. The present results show the applicability of scanning dielectric microscopy to nanoscale filamentous protein complexes and to general 3D macromolecular protein geometries, thus opening new avenues to study the relationship between the dielectric response and protein structure and function.",

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T1 - Dielectric constant of flagellin proteins measured by scanning dielectric microscopy

AU - Lozano, Helena

AU - Fabregas, Rene

AU - Blanco-Cabra, Núria

AU - Millán-Solsona, Rubén

AU - Torrents, Eduard

AU - Fumagalli, Laura

AU - Gomila, Gabriel

PY - 2018/10/28

Y1 - 2018/10/28

N2 - The dielectric constant of flagellin proteins in flagellar bacterial filaments ∼10-20 nm in diameter is measured using scanning dielectric microscopy. We obtained for two different bacterial species (Shewanella oneidensis MR-1 and Pseudomonas aeruginosa PAO1) similar relative dielectric constant values ϵSo = 4.3 ± 0.6 and ϵPa = 4.5 ± 0.7, respectively, despite their different structure and amino acid sequence. The present results show the applicability of scanning dielectric microscopy to nanoscale filamentous protein complexes and to general 3D macromolecular protein geometries, thus opening new avenues to study the relationship between the dielectric response and protein structure and function.

AB - The dielectric constant of flagellin proteins in flagellar bacterial filaments ∼10-20 nm in diameter is measured using scanning dielectric microscopy. We obtained for two different bacterial species (Shewanella oneidensis MR-1 and Pseudomonas aeruginosa PAO1) similar relative dielectric constant values ϵSo = 4.3 ± 0.6 and ϵPa = 4.5 ± 0.7, respectively, despite their different structure and amino acid sequence. The present results show the applicability of scanning dielectric microscopy to nanoscale filamentous protein complexes and to general 3D macromolecular protein geometries, thus opening new avenues to study the relationship between the dielectric response and protein structure and function.

UR - https://www.scopus.com/pages/publications/85055075024

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DO - 10.1039/c8nr06190d

M3 - Article

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SN - 2040-3364

VL - 10

SP - 19188

EP - 19194

JO - Nanoscale

JF - Nanoscale

IS - 40

ER -

Dielectric constant of flagellin proteins measured by scanning dielectric microscopy

Abstract

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