Dielectric constant of flagellin proteins measured by scanning dielectric microscopy

Helena Lozano, Rene Fabregas, Núria Blanco-Cabra, Rubén Millán-Solsona, Eduard Torrents, Laura Fumagalli, Gabriel Gomila

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The dielectric constant of flagellin proteins in flagellar bacterial filaments ∼10-20 nm in diameter is measured using scanning dielectric microscopy. We obtained for two different bacterial species (Shewanella oneidensis MR-1 and Pseudomonas aeruginosa PAO1) similar relative dielectric constant values ϵSo = 4.3 ± 0.6 and ϵPa = 4.5 ± 0.7, respectively, despite their different structure and amino acid sequence. The present results show the applicability of scanning dielectric microscopy to nanoscale filamentous protein complexes and to general 3D macromolecular protein geometries, thus opening new avenues to study the relationship between the dielectric response and protein structure and function.

Original languageEnglish
Pages (from-to)19188-19194
Number of pages7
JournalNanoscale
Volume10
Issue number40
DOIs
StatePublished - Oct 28 2018
Externally publishedYes

Fingerprint

Dive into the research topics of 'Dielectric constant of flagellin proteins measured by scanning dielectric microscopy'. Together they form a unique fingerprint.

Cite this