TY - JOUR
T1 - Di- and Triblock siRNA-PEG Copolymers
T2 - PEG Density Effect of Polyelectrolyte Complexes on Cellular Uptake and Gene Silencing Efficiency
AU - Lee, Soo Hyeon
AU - Mok, Hyejung
AU - Park, Tae Gwan
PY - 2011/3/10
Y1 - 2011/3/10
N2 - Di- and triblock siRNA/PEG copolymers were synthesized and complexed with cationic SLN for assessing their gene silencing efficiency as a function of PEG density. A sssiRNA and a sassiRNA were separately conjugated with PEG via a disulfide linkage. AB-type diblock and ABA-type triblock copolymers were successfully prepared by stoichiometric hybridization of sssiRNA-PEG conjugate with sassiRNA and sassiRNA-PEG conjugate, respectively. The resultant di- and triblock copolymers were characterized by means of GPC and gel electrophoresis. The serum stability of siRNA in the copolymers was enhanced as compared to that of naked siRNA. Using cationic SLN as a model carrier, the PEGylation density effect of the siRNA-PEG/SLN complexes on gene silencing and cellular uptake was analyzed.
AB - Di- and triblock siRNA/PEG copolymers were synthesized and complexed with cationic SLN for assessing their gene silencing efficiency as a function of PEG density. A sssiRNA and a sassiRNA were separately conjugated with PEG via a disulfide linkage. AB-type diblock and ABA-type triblock copolymers were successfully prepared by stoichiometric hybridization of sssiRNA-PEG conjugate with sassiRNA and sassiRNA-PEG conjugate, respectively. The resultant di- and triblock copolymers were characterized by means of GPC and gel electrophoresis. The serum stability of siRNA in the copolymers was enhanced as compared to that of naked siRNA. Using cationic SLN as a model carrier, the PEGylation density effect of the siRNA-PEG/SLN complexes on gene silencing and cellular uptake was analyzed.
KW - Conjugates
KW - Gene silencing
KW - Intracellular uptake
KW - Poly(ethylene glycol) (PEG)
KW - Small interfering RNA (siRNA)
UR - http://www.scopus.com/inward/record.url?scp=79952075437&partnerID=8YFLogxK
U2 - 10.1002/mabi.201000347
DO - 10.1002/mabi.201000347
M3 - Article
C2 - 21080384
AN - SCOPUS:79952075437
SN - 1616-5187
VL - 11
SP - 410
EP - 418
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
IS - 3
ER -