Development of resonance ionization spectroscopy for genome mapping and DNA sequencing using stable isotopes as DNA labels

K. Bruce Jacobson; Heinrich F. Arlinghaus; Mitchel J. Doktycz; Richard A. Sachleben; Gilbert M. Brown; Frank W. Larimer

doi:10.1117/12.146716

Development of resonance ionization spectroscopy for genome mapping and DNA sequencing using stable isotopes as DNA labels

K. Bruce Jacobson, Heinrich F. Arlinghaus, Mitchel J. Doktycz, Richard A. Sachleben, Gilbert M. Brown, Frank W. Larimer

Biosciences Division

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

Stable isotopes can be used as alternatives to radioisotopes or fluorescent DNA labels. After the labeled DNA has been banded on electrophoresis gels the stable isotopes may be located by scanning the gel with resonance ionization spectroscopy. Methods for synthesizing appropriate labels and for detecting isotopes of iron and tin directly on polyacrylamide gels are described.

Original language	English
Pages (from-to)	27-36
Number of pages	10
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	1891
DOIs	https://doi.org/10.1117/12.146716
State	Published - Jun 24 1993
Event	Advances in DNA Sequencing Technology 1993 - Los Angeles, United States Duration: Jan 17 1993 → Jan 22 1993

Funding

This research was sponsored by the Office of Health and Environmental Sciences of the U. S. Department of Energy under contract DE-ACO5-84OR21400 with Martin Marietta Energy Systems, Inc. and contracts DEFGO5-91ER81235 and DE-FG-05091ER81235 with Atom Sciences, Inc. and No. 1R43CA54627-O1 from NIH to Atom Sciences, Inc. valuable contributions to this research. This research was sponsored by the Office of

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title = "Development of resonance ionization spectroscopy for genome mapping and DNA sequencing using stable isotopes as DNA labels",

abstract = "Stable isotopes can be used as alternatives to radioisotopes or fluorescent DNA labels. After the labeled DNA has been banded on electrophoresis gels the stable isotopes may be located by scanning the gel with resonance ionization spectroscopy. Methods for synthesizing appropriate labels and for detecting isotopes of iron and tin directly on polyacrylamide gels are described.",

author = "Jacobson, {K. Bruce} and Arlinghaus, {Heinrich F.} and Doktycz, {Mitchel J.} and Sachleben, {Richard A.} and Brown, {Gilbert M.} and Larimer, {Frank W.}",

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language = "English",

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Development of resonance ionization spectroscopy for genome mapping and DNA sequencing using stable isotopes as DNA labels. / Jacobson, K. Bruce; Arlinghaus, Heinrich F.; Doktycz, Mitchel J. et al.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 1891, 24.06.1993, p. 27-36.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Development of resonance ionization spectroscopy for genome mapping and DNA sequencing using stable isotopes as DNA labels

AU - Jacobson, K. Bruce

AU - Arlinghaus, Heinrich F.

AU - Doktycz, Mitchel J.

AU - Sachleben, Richard A.

AU - Brown, Gilbert M.

AU - Larimer, Frank W.

PY - 1993/6/24

Y1 - 1993/6/24

N2 - Stable isotopes can be used as alternatives to radioisotopes or fluorescent DNA labels. After the labeled DNA has been banded on electrophoresis gels the stable isotopes may be located by scanning the gel with resonance ionization spectroscopy. Methods for synthesizing appropriate labels and for detecting isotopes of iron and tin directly on polyacrylamide gels are described.

AB - Stable isotopes can be used as alternatives to radioisotopes or fluorescent DNA labels. After the labeled DNA has been banded on electrophoresis gels the stable isotopes may be located by scanning the gel with resonance ionization spectroscopy. Methods for synthesizing appropriate labels and for detecting isotopes of iron and tin directly on polyacrylamide gels are described.

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U2 - 10.1117/12.146716

DO - 10.1117/12.146716

M3 - Conference article

AN - SCOPUS:8944230729

SN - 0277-786X

VL - 1891

SP - 27

EP - 36

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Advances in DNA Sequencing Technology 1993

Y2 - 17 January 1993 through 22 January 1993

ER -

Development of resonance ionization spectroscopy for genome mapping and DNA sequencing using stable isotopes as DNA labels

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