Development of nanosensors and bioprobes

Tuan Vo-Dinh, Guy D. Griffin, Jean Pierre Alarie, Brian Cullum, Bobby Sumpter, Donald Noid

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

We describe the development and application of nanosensors having bioreceptor probes for bioanalysis. The nanoprobes were fabricated with optical fibers pulled down to tips having distal end sizes of approximately 30-60 nm. The use of two different types of receptors was investigated. Fiberoptic nanoprobes were covalently bound either with bioreceptors, such as antibodies, or with other receptors, such as cyclodextrins that are selective for the size and chemical structure of the analyte molecules. Theoretical calculations were performed to model the binding of beta-cyclodextrin with pyrene and 5,6-benzoquinoline, and to illustrate the possibility of comparing experimental data with theoretical data. The antibody-based nanoprobe was used for in situ measurements of benzopyrene tetrol in single cells. The performance of the nanosensor is illustrated by intracellular measurements performed on a rat liver epithelial cell line (Clone 9) used as the model cell system. The usefulness and potential of these nanotechnology-based biosensors in biological research and applications are discussed.

Original languageEnglish
Pages (from-to)17-27
Number of pages11
JournalJournal of Nanoparticle Research
Volume2
Issue number1
DOIs
StatePublished - 2000

Keywords

  • Antibody
  • Benzopyrene tetrol
  • Biosensor
  • Cancer
  • Cyclodextrin
  • Nanoprobe
  • Nanosensor
  • Nanotechnology

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