Development and Application of DropletProbe Mass Spectrometry for Examining Biodistribution of Therapeutics

Bingming Chen; Marissa Vavrek; Mark T. Cancilla; Vilmos Kertesz

doi:10.1007/978-1-0716-2030-4_12

Development and Application of DropletProbe Mass Spectrometry for Examining Biodistribution of Therapeutics

Bingming Chen, Marissa Vavrek, Mark T. Cancilla, Vilmos Kertesz

Bioanalytical Mass Spectrometry

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Scopus citations

Abstract

dropletProbe mass spectrometry is a novel technique for molecular characterization of surfaces. It can be used for rapid ex vivo analysis of therapeutics from thin animal tissue sections and has been shown to improve understanding of a drug’s absorption, distribution, metabolism and excretion (ADME) properties. Here, we describe the tissue distribution analysis of diclofenac from a dosed whole-body mouse thin tissue section using a dropletProbe mass spectrometry system.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	171-180
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-0716-2030-4_12
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2437
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Funding

The work of V.K. was supported by the US Department of Energy, Office of Science, Biological and Environmental Research, Bioima-ging Science Program. The authors acknowledge Alain Creissen and Sloan Tilley (HTX Technology) and Matt Orcutt (Resolution Labs) for providing technical support of dropletProbe MS. We acknowledge Scott Fauty (Merck & Co., Inc., Kenilworth, NJ, USA) for performing animal experiments.

Keywords

Diclofenac
DropletProbe
Mass spectrometry
Metabolite identification
Tissue distribution
Whole-body analysis

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10.1007/978-1-0716-2030-4_12

Cite this

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title = "Development and Application of DropletProbe Mass Spectrometry for Examining Biodistribution of Therapeutics",

abstract = "dropletProbe mass spectrometry is a novel technique for molecular characterization of surfaces. It can be used for rapid ex vivo analysis of therapeutics from thin animal tissue sections and has been shown to improve understanding of a drug{\textquoteright}s absorption, distribution, metabolism and excretion (ADME) properties. Here, we describe the tissue distribution analysis of diclofenac from a dosed whole-body mouse thin tissue section using a dropletProbe mass spectrometry system.",

keywords = "Diclofenac, DropletProbe, Mass spectrometry, Metabolite identification, Tissue distribution, Whole-body analysis",

author = "Bingming Chen and Marissa Vavrek and Cancilla, \{Mark T.\} and Vilmos Kertesz",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

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AU - Chen, Bingming

AU - Vavrek, Marissa

AU - Cancilla, Mark T.

AU - Kertesz, Vilmos

PY - 2022

Y1 - 2022

N2 - dropletProbe mass spectrometry is a novel technique for molecular characterization of surfaces. It can be used for rapid ex vivo analysis of therapeutics from thin animal tissue sections and has been shown to improve understanding of a drug’s absorption, distribution, metabolism and excretion (ADME) properties. Here, we describe the tissue distribution analysis of diclofenac from a dosed whole-body mouse thin tissue section using a dropletProbe mass spectrometry system.

AB - dropletProbe mass spectrometry is a novel technique for molecular characterization of surfaces. It can be used for rapid ex vivo analysis of therapeutics from thin animal tissue sections and has been shown to improve understanding of a drug’s absorption, distribution, metabolism and excretion (ADME) properties. Here, we describe the tissue distribution analysis of diclofenac from a dosed whole-body mouse thin tissue section using a dropletProbe mass spectrometry system.

KW - Diclofenac

KW - DropletProbe

KW - Mass spectrometry

KW - Metabolite identification

KW - Tissue distribution

KW - Whole-body analysis

UR - https://www.scopus.com/pages/publications/85121511924

U2 - 10.1007/978-1-0716-2030-4_12

DO - 10.1007/978-1-0716-2030-4_12

M3 - Chapter

C2 - 34902148

AN - SCOPUS:85121511924

T3 - Methods in Molecular Biology

SP - 171

EP - 180

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Development and Application of DropletProbe Mass Spectrometry for Examining Biodistribution of Therapeutics

Abstract

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Keywords

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