Development and Application of DropletProbe Mass Spectrometry for Examining Biodistribution of Therapeutics

Bingming Chen, Marissa Vavrek, Mark T. Cancilla, Vilmos Kertesz

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

dropletProbe mass spectrometry is a novel technique for molecular characterization of surfaces. It can be used for rapid ex vivo analysis of therapeutics from thin animal tissue sections and has been shown to improve understanding of a drug’s absorption, distribution, metabolism and excretion (ADME) properties. Here, we describe the tissue distribution analysis of diclofenac from a dosed whole-body mouse thin tissue section using a dropletProbe mass spectrometry system.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages171-180
Number of pages10
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2437
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Funding

The work of V.K. was supported by the US Department of Energy, Office of Science, Biological and Environmental Research, Bioima-ging Science Program. The authors acknowledge Alain Creissen and Sloan Tilley (HTX Technology) and Matt Orcutt (Resolution Labs) for providing technical support of dropletProbe MS. We acknowledge Scott Fauty (Merck & Co., Inc., Kenilworth, NJ, USA) for performing animal experiments.

FundersFunder number
U.S. Department of Energy
Office of Science
Biological and Environmental Research

    Keywords

    • Diclofenac
    • DropletProbe
    • Mass spectrometry
    • Metabolite identification
    • Tissue distribution
    • Whole-body analysis

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