TY - GEN
T1 - Developing in vitro models of the sub-retinal microenvironment
AU - Vargis, Elizabeth
AU - Foster, Carmen
AU - Peterson, Cristen B.
AU - Morrell-Falvey, Jennifer L.
AU - Retterer, Scott T.
AU - Collier, C. Patrick
PY - 2013
Y1 - 2013
N2 - Physiologically-relevant in vitro models of retinal disease are necessary for understanding the complex interactions of oxidative stress, molecular signaling and physical contact between cells and their local environment. In this study, microfluidic devices and microcontact printing are used to mimic in vivo conditions of the sub-retinal microenvironment and the effects of oxidative stress and atrophy on protein expression by retinal pigment epithelial cells. The results demonstrate that differences in RNA and protein expression due to oxidative stress and loss of function can be observed from cells within microfluidic devices and in micropatterned patches. These findings indicate that nano- and microstructured materials can be used to interrogate normal and malignant retinal cell growth.
AB - Physiologically-relevant in vitro models of retinal disease are necessary for understanding the complex interactions of oxidative stress, molecular signaling and physical contact between cells and their local environment. In this study, microfluidic devices and microcontact printing are used to mimic in vivo conditions of the sub-retinal microenvironment and the effects of oxidative stress and atrophy on protein expression by retinal pigment epithelial cells. The results demonstrate that differences in RNA and protein expression due to oxidative stress and loss of function can be observed from cells within microfluidic devices and in micropatterned patches. These findings indicate that nano- and microstructured materials can be used to interrogate normal and malignant retinal cell growth.
KW - microcontact printing
KW - microfluidics
KW - protein absorption
KW - retinal pigment epithelial cells
UR - http://www.scopus.com/inward/record.url?scp=84887812076&partnerID=8YFLogxK
U2 - 10.1109/BSEC.2013.6618483
DO - 10.1109/BSEC.2013.6618483
M3 - Conference contribution
AN - SCOPUS:84887812076
SN - 9781479921188
T3 - Proceedings of the 2013 4th Annual ORNL Biomedical Sciences and Engineering Conference: Collaborative Biomedical Innovations, BSEC 2013
BT - Proceedings of the 2013 4th Annual ORNL Biomedical Sciences and Engineering Conference
T2 - 2013 4th Annual ORNL Biomedical Sciences and Engineering Conference: Collaborative Biomedical Innovations, BSEC 2013
Y2 - 21 May 2013 through 23 May 2013
ER -