Developing in vitro models of the sub-retinal microenvironment

Elizabeth Vargis, Carmen Foster, Cristen B. Peterson, Jennifer L. Morrell-Falvey, Scott T. Retterer, C. Patrick Collier

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Physiologically-relevant in vitro models of retinal disease are necessary for understanding the complex interactions of oxidative stress, molecular signaling and physical contact between cells and their local environment. In this study, microfluidic devices and microcontact printing are used to mimic in vivo conditions of the sub-retinal microenvironment and the effects of oxidative stress and atrophy on protein expression by retinal pigment epithelial cells. The results demonstrate that differences in RNA and protein expression due to oxidative stress and loss of function can be observed from cells within microfluidic devices and in micropatterned patches. These findings indicate that nano- and microstructured materials can be used to interrogate normal and malignant retinal cell growth.

Original languageEnglish
Title of host publicationProceedings of the 2013 4th Annual ORNL Biomedical Sciences and Engineering Conference
Subtitle of host publicationCollaborative Biomedical Innovations, BSEC 2013
DOIs
StatePublished - 2013
Event2013 4th Annual ORNL Biomedical Sciences and Engineering Conference: Collaborative Biomedical Innovations, BSEC 2013 - Oak Ridge, TN, United States
Duration: May 21 2013May 23 2013

Publication series

NameProceedings of the 2013 4th Annual ORNL Biomedical Sciences and Engineering Conference: Collaborative Biomedical Innovations, BSEC 2013

Conference

Conference2013 4th Annual ORNL Biomedical Sciences and Engineering Conference: Collaborative Biomedical Innovations, BSEC 2013
Country/TerritoryUnited States
CityOak Ridge, TN
Period05/21/1305/23/13

Keywords

  • microcontact printing
  • microfluidics
  • protein absorption
  • retinal pigment epithelial cells

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