TY - GEN
T1 - Determining toxicant bioavailability using a constitutively bioluminescent human cell line
AU - Close, Dan M.
AU - Hahn, Ruth E.
AU - Ripp, Steven A.
AU - Sayler, Gary S.
PY - 2011
Y1 - 2011
N2 - Despite excessive expenditures by governments and private corporations, the toxicological effects of many chemicals and human relevant compounds are not well understood. Current methods for screening the toxicological effects of these chemicals are expensive, time consuming, and have been proven to produce results that must be interpreted with caution when extrapolating to human bioavailability and toxic impact. Here we demonstrate the use of a constitutively bioluminescent human HEK293 cell line expressing the genes of the bacterial luciferase gene cassette as a simple, low cost method for initial screening of toxic compounds. The autonomous nature of the bacterial bioluminescence cassette negates concerns over substrate addition, while providing a facile means for detection of reduced cellular growth or metabolism as a biomarker for toxic chemical exposure.
AB - Despite excessive expenditures by governments and private corporations, the toxicological effects of many chemicals and human relevant compounds are not well understood. Current methods for screening the toxicological effects of these chemicals are expensive, time consuming, and have been proven to produce results that must be interpreted with caution when extrapolating to human bioavailability and toxic impact. Here we demonstrate the use of a constitutively bioluminescent human HEK293 cell line expressing the genes of the bacterial luciferase gene cassette as a simple, low cost method for initial screening of toxic compounds. The autonomous nature of the bacterial bioluminescence cassette negates concerns over substrate addition, while providing a facile means for detection of reduced cellular growth or metabolism as a biomarker for toxic chemical exposure.
UR - http://www.scopus.com/inward/record.url?scp=79959891828&partnerID=8YFLogxK
U2 - 10.1109/BSEC.2011.5872321
DO - 10.1109/BSEC.2011.5872321
M3 - Conference contribution
AN - SCOPUS:79959891828
SN - 9781612844107
T3 - Proceedings of the 2011 Biomedical Sciences and Engineering Conference: Image Informatics and Analytics in Biomedicine, BSEC 2011
BT - Proceedings of the 2011 Biomedical Sciences and Engineering Conference
T2 - 2011 Biomedical Sciences and Engineering Conference: Image Informatics and Analytics in Biomedicine, BSEC 2011
Y2 - 15 March 2011 through 17 March 2011
ER -