Determining the Syringyl/Guaiacyl Lignin Ratio in the Vessel and Fiber Cell Walls of Transgenic Populus Plants

Allison K. Tolbert; Tao Ma; Udaya C. Kalluri; Arthur J. Ragauskas

doi:10.1021/acs.energyfuels.6b00560

Determining the Syringyl/Guaiacyl Lignin Ratio in the Vessel and Fiber Cell Walls of Transgenic Populus Plants

Allison K. Tolbert, Tao Ma, Udaya C. Kalluri, Arthur J. Ragauskas

Plant Systems Biology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Observation of the spatial lignin distribution throughout the plant cell wall provides insight into the physicochemical characteristics of lignocellulosic biomass. The distribution of syringyl (S) and guaiacyl (G) lignin in cell walls of a genetically modified Populus deltoides and its corresponding empty vector control were analyzed with time-of-flight secondary ion mass spectrometry (ToF-SIMS) and then mapped to determine the S/G lignin ratio of the sample surface and specific regions of interest (ROIs). The surface characterizations of transgenic cross-sections within 1 cm vertical distance of each other on the stem possess similar S/G lignin ratios. The analysis of the ROIs determined that there was a 50% decrease in the S/G lignin ratio of the transgenic xylem fiber cell walls.

Original language	English
Pages (from-to)	5716-5720
Number of pages	5
Journal	Energy and Fuels
Volume	30
Issue number	7
DOIs	https://doi.org/10.1021/acs.energyfuels.6b00560
State	Published - Jul 21 2016

Funding

This manuscript has been authored by University of Tennessee (UT)-Battelle, LLC under Contract DE-AC05-00OR22725 with the U.S. Department of Energy. The BioEnergy Science Center is supported by the Office of Biological and Environmental Research in the U.S. Department of Energy. Mass spectrometry analysis was carried out by the U.S. Department of Energy Office of Biological and Environmental Research supported by the Bioenergy Research Center proteomics pipeline. The publisher, by accepting the article for publication, acknowledges that the United States Government retains a non-exclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this manuscript or allow others to do so for United States Government purposes. The U.S. Department of Energy will provide public access to these results of federally sponsored research in accordance with the DOE Public Access Plan (http://energy.gov/downloads/doe-public-access-plan). Allison K. Tolbert is grateful for the financial support from the Paper Science and Enegineering (PSE) fellowship program at the Renewable Bioproducts Institute at Georgia Institute of Technology.

Funders	Funder number
Bioenergy Research Center proteomics pipeline
DOE Public Access Plan
Paper Science and Enegineering
United States Government
U.S. Department of Energy
Biological and Environmental Research
Georgia Institute of Technology
University of Tennessee	DE-AC05-00OR22725

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10.1021/acs.energyfuels.6b00560

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title = "Determining the Syringyl/Guaiacyl Lignin Ratio in the Vessel and Fiber Cell Walls of Transgenic Populus Plants",

abstract = "Observation of the spatial lignin distribution throughout the plant cell wall provides insight into the physicochemical characteristics of lignocellulosic biomass. The distribution of syringyl (S) and guaiacyl (G) lignin in cell walls of a genetically modified Populus deltoides and its corresponding empty vector control were analyzed with time-of-flight secondary ion mass spectrometry (ToF-SIMS) and then mapped to determine the S/G lignin ratio of the sample surface and specific regions of interest (ROIs). The surface characterizations of transgenic cross-sections within 1 cm vertical distance of each other on the stem possess similar S/G lignin ratios. The analysis of the ROIs determined that there was a 50% decrease in the S/G lignin ratio of the transgenic xylem fiber cell walls.",

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AU - Ragauskas, Arthur J.

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AB - Observation of the spatial lignin distribution throughout the plant cell wall provides insight into the physicochemical characteristics of lignocellulosic biomass. The distribution of syringyl (S) and guaiacyl (G) lignin in cell walls of a genetically modified Populus deltoides and its corresponding empty vector control were analyzed with time-of-flight secondary ion mass spectrometry (ToF-SIMS) and then mapped to determine the S/G lignin ratio of the sample surface and specific regions of interest (ROIs). The surface characterizations of transgenic cross-sections within 1 cm vertical distance of each other on the stem possess similar S/G lignin ratios. The analysis of the ROIs determined that there was a 50% decrease in the S/G lignin ratio of the transgenic xylem fiber cell walls.

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Determining the Syringyl/Guaiacyl Lignin Ratio in the Vessel and Fiber Cell Walls of Transgenic Populus Plants

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