Detection and differentiation of biological species using microcalorimetric spectroscopy

E. T. Arakawa; N. V. Lavrik; S. Rajic; P. G. Datskos

doi:10.1016/S0304-3991(03)00074-3

Detection and differentiation of biological species using microcalorimetric spectroscopy

E. T. Arakawa, N. V. Lavrik, S. Rajic, P. G. Datskos

Nanofabrication Research Laboratory

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

We report on the application of infrared (IR) microcalorimetric spectroscopy (μ-CalSpec) to the identification and detection of trace amounts of biological species. Our approach combines principles of photothermal IR spectroscopy with ultrasensitive microcantilever (MC) thermal detectors. We have obtained photothermal IR spectra for DNA and RNA bases and for Bacillus Cereus (an anthrax simulant) in the wavelength range of 2.5-14.5μm (4000-690cm^-1). The measurements are accomplished by absorbing biological materials directly on a MC thermal detector. The main advantage of the developed μ-CalSpec is its unprecedented sensitivity as compared to any of the previously explored IR techniques, including FTIR and photothermal FTIR methods. Our results demonstrate that <10^-9g of a biological sample is sufficient to obtain its characteristic μ-CalSpec spectrum that contains information-rich chemical (vibrational) signatures. This opens up a new opportunity to create inexpensive high-throughput analytical systems for biochemical detection.

Original language	English
Pages (from-to)	459-465
Number of pages	7
Journal	Ultramicroscopy
Volume	97
Issue number	1-4
DOIs	https://doi.org/10.1016/S0304-3991(03)00074-3
State	Published - 2003

Funding

We would like to acknowledge support from the Defense Advanced Research Projects Agency, the National Science Foundation and DOE. Oak Ridge National Laboratory is operated for the US Department of Energy by UT-Battelle under contract DE-AC05-96OR22464. We would also like to thank B.N. Khare, NASA Ames Research Center, Moffett Field, CA, and M.E. Milham, Edgewood research, Development and Engineering Center, Aberdeen Proving Ground, MD for helping to provide the B. Cereus spores used in this study.

Funders	Funder number
National Science Foundation
U.S. Department of Energy
Defense Advanced Research Projects Agency
UT-Battelle	DE-AC05-96OR22464

Keywords

Anthrax
CalSpec
DNA
IR spectroscopy
Microcantilever
RNA

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0304-3991(03)00074-3

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title = "Detection and differentiation of biological species using microcalorimetric spectroscopy",

abstract = "We report on the application of infrared (IR) microcalorimetric spectroscopy (μ-CalSpec) to the identification and detection of trace amounts of biological species. Our approach combines principles of photothermal IR spectroscopy with ultrasensitive microcantilever (MC) thermal detectors. We have obtained photothermal IR spectra for DNA and RNA bases and for Bacillus Cereus (an anthrax simulant) in the wavelength range of 2.5-14.5μm (4000-690cm-1). The measurements are accomplished by absorbing biological materials directly on a MC thermal detector. The main advantage of the developed μ-CalSpec is its unprecedented sensitivity as compared to any of the previously explored IR techniques, including FTIR and photothermal FTIR methods. Our results demonstrate that <10-9g of a biological sample is sufficient to obtain its characteristic μ-CalSpec spectrum that contains information-rich chemical (vibrational) signatures. This opens up a new opportunity to create inexpensive high-throughput analytical systems for biochemical detection.",

keywords = "Anthrax, CalSpec, DNA, IR spectroscopy, Microcantilever, RNA",

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AU - Arakawa, E. T.

AU - Lavrik, N. V.

AU - Rajic, S.

AU - Datskos, P. G.

PY - 2003

Y1 - 2003

N2 - We report on the application of infrared (IR) microcalorimetric spectroscopy (μ-CalSpec) to the identification and detection of trace amounts of biological species. Our approach combines principles of photothermal IR spectroscopy with ultrasensitive microcantilever (MC) thermal detectors. We have obtained photothermal IR spectra for DNA and RNA bases and for Bacillus Cereus (an anthrax simulant) in the wavelength range of 2.5-14.5μm (4000-690cm-1). The measurements are accomplished by absorbing biological materials directly on a MC thermal detector. The main advantage of the developed μ-CalSpec is its unprecedented sensitivity as compared to any of the previously explored IR techniques, including FTIR and photothermal FTIR methods. Our results demonstrate that <10-9g of a biological sample is sufficient to obtain its characteristic μ-CalSpec spectrum that contains information-rich chemical (vibrational) signatures. This opens up a new opportunity to create inexpensive high-throughput analytical systems for biochemical detection.

AB - We report on the application of infrared (IR) microcalorimetric spectroscopy (μ-CalSpec) to the identification and detection of trace amounts of biological species. Our approach combines principles of photothermal IR spectroscopy with ultrasensitive microcantilever (MC) thermal detectors. We have obtained photothermal IR spectra for DNA and RNA bases and for Bacillus Cereus (an anthrax simulant) in the wavelength range of 2.5-14.5μm (4000-690cm-1). The measurements are accomplished by absorbing biological materials directly on a MC thermal detector. The main advantage of the developed μ-CalSpec is its unprecedented sensitivity as compared to any of the previously explored IR techniques, including FTIR and photothermal FTIR methods. Our results demonstrate that <10-9g of a biological sample is sufficient to obtain its characteristic μ-CalSpec spectrum that contains information-rich chemical (vibrational) signatures. This opens up a new opportunity to create inexpensive high-throughput analytical systems for biochemical detection.

KW - Anthrax

KW - CalSpec

KW - DNA

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KW - Microcantilever

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ER -

Detection and differentiation of biological species using microcalorimetric spectroscopy

Abstract

Funding

Keywords

UN SDGs

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