Detection and analysis of polymerase chain reaction products by mass spectrometry

Gregory B. Hurst; Mitchel J. Doktycz; Philip F. Britt; Arpad A. Vass; Michelle V. Buchanan

doi:10.1117/12.274345

Detection and analysis of polymerase chain reaction products by mass spectrometry

Gregory B. Hurst, Mitchel J. Doktycz, Philip F. Britt, Arpad A. Vass, Michelle V. Buchanan

Research output: Contribution to journal › Conference article › peer-review

Abstract

The use of matrix-assisted laser desorption/ionization (MALDI) and time-of-flight mass spectrometry for analysis and detection of deoxyribonucleic acid (DNA) polymers and polymerase chain reaction products was discussed. The measurement of DNA polymers in a specific size range was found to be necessary for fine-structure genome analysis. The use of the tailored cleanup techniques provided improved analysis of DNA by providing better sensitivity and resolution at the higher molecular weights of proteins.

Original language	English
Pages (from-to)	120-128
Number of pages	9
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	2985
DOIs	https://doi.org/10.1117/12.274345
State	Published - 1997
Event	Ultrasensitive Biochemical Diagnostics II - San Jose, CA, United States Duration: Feb 10 1997 → Feb 12 1997

Keywords

DNA
Laser desorption
MALDI
Mass spectrometry

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10.1117/12.274345

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@article{242a22dc5000451cba3be9b699ed824f,

title = "Detection and analysis of polymerase chain reaction products by mass spectrometry",

abstract = "The use of matrix-assisted laser desorption/ionization (MALDI) and time-of-flight mass spectrometry for analysis and detection of deoxyribonucleic acid (DNA) polymers and polymerase chain reaction products was discussed. The measurement of DNA polymers in a specific size range was found to be necessary for fine-structure genome analysis. The use of the tailored cleanup techniques provided improved analysis of DNA by providing better sensitivity and resolution at the higher molecular weights of proteins.",

keywords = "DNA, Laser desorption, MALDI, Mass spectrometry",

author = "Hurst, \{Gregory B.\} and Doktycz, \{Mitchel J.\} and Britt, \{Philip F.\} and Vass, \{Arpad A.\} and Buchanan, \{Michelle V.\}",

year = "1997",

doi = "10.1117/12.274345",

language = "English",

volume = "2985",

pages = "120--128",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "Society of Photo-Optical Instrumentation Engineers",

note = "Ultrasensitive Biochemical Diagnostics II ; Conference date: 10-02-1997 Through 12-02-1997",

}

TY - JOUR

T1 - Detection and analysis of polymerase chain reaction products by mass spectrometry

AU - Hurst, Gregory B.

AU - Doktycz, Mitchel J.

AU - Britt, Philip F.

AU - Vass, Arpad A.

AU - Buchanan, Michelle V.

PY - 1997

Y1 - 1997

N2 - The use of matrix-assisted laser desorption/ionization (MALDI) and time-of-flight mass spectrometry for analysis and detection of deoxyribonucleic acid (DNA) polymers and polymerase chain reaction products was discussed. The measurement of DNA polymers in a specific size range was found to be necessary for fine-structure genome analysis. The use of the tailored cleanup techniques provided improved analysis of DNA by providing better sensitivity and resolution at the higher molecular weights of proteins.

AB - The use of matrix-assisted laser desorption/ionization (MALDI) and time-of-flight mass spectrometry for analysis and detection of deoxyribonucleic acid (DNA) polymers and polymerase chain reaction products was discussed. The measurement of DNA polymers in a specific size range was found to be necessary for fine-structure genome analysis. The use of the tailored cleanup techniques provided improved analysis of DNA by providing better sensitivity and resolution at the higher molecular weights of proteins.

KW - DNA

KW - Laser desorption

KW - MALDI

KW - Mass spectrometry

UR - https://www.scopus.com/pages/publications/0031389496

U2 - 10.1117/12.274345

DO - 10.1117/12.274345

M3 - Conference article

AN - SCOPUS:0031389496

SN - 0277-786X

VL - 2985

SP - 120

EP - 128

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Ultrasensitive Biochemical Diagnostics II

Y2 - 10 February 1997 through 12 February 1997

ER -

Detection and analysis of polymerase chain reaction products by mass spectrometry

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Keywords

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