Dependence on the phospholipid polyunsaturated fatty acids of the oxidative injury of isolated cardiomyocytes

Isabelle Durot; Lisa Devillard; Cindy Tissier; David Vandroux; Sophie Voisin; Sabir Jaquir; Luc Rochette; Pierre Athias

doi:10.1080/10715760500509165

Dependence on the phospholipid polyunsaturated fatty acids of the oxidative injury of isolated cardiomyocytes

Isabelle Durot, Lisa Devillard, Cindy Tissier, David Vandroux, Sophie Voisin, Sabir Jaquir, Luc Rochette, Pierre Athias

Data and Software Engineering

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2 Scopus citations

Abstract

We investigated the influence of PUFA in phospholipids (PL) on the functional characteristics of cultured cardiomyocytes (CM) in basal conditions and during free radical (FR) stress provoked either by the xanthine/xanthine oxidase (X/XO) system or by a (9Z, 11E, 13 (S), 15Z)-13-hydroperoxyoctadecatrienoic acid (13-HpOTrE). CM were grown in media containing either n - 3 (eicosapentaenoic acid, EPA, and docosahexaenoic acid, DHA) or n - 6 (arachidonic acid, AA). These two groups of CM displayed different PUFA n -6/n - 3 ratio in PL. However, their basal electromechanical characteristics were similar. The X/XO system drastically altered CM functions, without difference between the two groups of CM. 13-HpOTrE caused a moderate and reversible depression in action potential parameters, which was dependent upon the PL PUFA, since the n - 3-enriched CM exhibited an earlier functional depression but faster recovery. Thus, the peroxidative damage of CM depended on a cross relationship between FR species and the PL PUFA composition.

Original language	English
Pages (from-to)	251-261
Number of pages	11
Journal	Free Radical Research
Volume	40
Issue number	3
DOIs	https://doi.org/10.1080/10715760500509165
State	Published - Mar 2006

Funding

This work was supported by grants from the French Ministry of Research, the Regional Council of Burgundy, the French Federation of Cardiology, the Cardiology Association of Burgundy and the French National League against Cancer. The authors are very grateful to Mr Jean-Pierre Iliou (Laboratoires Servier, Neuilly, France) for the kind gift of the hydroperox-ydecatrienoic acid. The authors are also grateful to Dr Paul Walker for proof reading.

Keywords

13-hydroperoxyoctadecatrienoic acid
Cell physiology
Free radicals
Polyunsaturated fatty acids
Rat cardiomyocytes
Xanthine/xanthine oxidase

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title = "Dependence on the phospholipid polyunsaturated fatty acids of the oxidative injury of isolated cardiomyocytes",

abstract = "We investigated the influence of PUFA in phospholipids (PL) on the functional characteristics of cultured cardiomyocytes (CM) in basal conditions and during free radical (FR) stress provoked either by the xanthine/xanthine oxidase (X/XO) system or by a (9Z, 11E, 13 (S), 15Z)-13-hydroperoxyoctadecatrienoic acid (13-HpOTrE). CM were grown in media containing either n - 3 (eicosapentaenoic acid, EPA, and docosahexaenoic acid, DHA) or n - 6 (arachidonic acid, AA). These two groups of CM displayed different PUFA n -6/n - 3 ratio in PL. However, their basal electromechanical characteristics were similar. The X/XO system drastically altered CM functions, without difference between the two groups of CM. 13-HpOTrE caused a moderate and reversible depression in action potential parameters, which was dependent upon the PL PUFA, since the n - 3-enriched CM exhibited an earlier functional depression but faster recovery. Thus, the peroxidative damage of CM depended on a cross relationship between FR species and the PL PUFA composition.",

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AU - Voisin, Sophie

AU - Jaquir, Sabir

AU - Rochette, Luc

AU - Athias, Pierre

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KW - Free radicals

KW - Polyunsaturated fatty acids

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Dependence on the phospholipid polyunsaturated fatty acids of the oxidative injury of isolated cardiomyocytes

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