Abstract
This paper aimed at studying a novel high biomass strain (HBS) of marine protist, Thraustochytrium striatum, for lignin valorization to lipids and carotenoids. In the screening of 15 aromatic compounds, T. striatum HBS can utilize most compounds while achieving the best growth on 3,4-dihydroxybenzoic acid (3,4-DHBA) and 4-hydroxybenzoic acid (4-HBA). The maximum cell mass concentration reached 2−3 g/L under 5 g/L of 3,4-DHBA or 2 g/L of 4-HBA, which was comparable to that under equivalent concentration of glucose. The long-chain fatty acids content was 13 % and 7 % with 3,4-DHBA and 4-HBA, respectively. The adoption of fed-batch fermentation mode improved the degradation of carbon source and prevented the inhibitory effect caused by overloading of aromatic compounds. Both final cellular fatty acids and carotenoids contents were doubled in the fermentation of 3,4-DHBA. T. striatum HBS exhibited limited lignin depolymerization (∼24 %) ability on technical lignins.
| Original language | English |
|---|---|
| Pages (from-to) | 13-17 |
| Number of pages | 5 |
| Journal | Process Biochemistry |
| Volume | 107 |
| DOIs | |
| State | Published - Aug 2021 |
Funding
The authors would like to thank Dr. Maobin Tu, Associate Professor in the Department of Biomedical, Chemical and Environmental Engineering at the University of Cincinnati for providing EOL LP, and Dr. Mark Thies, Dow Chemical Professor, Chemical & Biomolecular Engineering, Clemson University for providing KPL for our research. This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Keywords
- Aromatic compounds
- Carotenoids
- Fatty acids
- Fermentation
- Lignin bioconversion
- Thraustochytrium striatum