DbAMEPNI: A database of alanine mutagenic effects for protein-nucleic acid interactions

Ling Liu, Yi Xiong, Hongyun Gao, Dong Qing Wei, Julie C. Mitchell, Xiaolei Zhu

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Protein-nucleic acid interactions play essential roles in various biological activities such as gene regulation, transcription, DNA repair and DNA packaging. Understanding the effects of amino acid substitutions on protein-nucleic acid binding affinities can help elucidate the molecular mechanism of protein-nucleic acid recognition. Until now, no comprehensive and updated database of quantitative binding data on alanine mutagenic effects for protein-nucleic acid interactions is publicly accessible. Thus, we developed a new database of Alanine Mutagenic Effects for Protein-Nucleic Acid Interactions (dbAMEPNI). dbAMEPNI is a manually curated, literature-derived database, comprising over 577 alanine mutagenic data with experimentally determined binding affinities for protein-nucleic acid complexes. It contains several important parameters, such as dissociation constant (Kd), Gibbs free energy change (ΔΔ G), experimental conditions and structural parameters of mutant residues. In addition, the database provides an extended dataset of 282 single alanine mutations with only qualitative data (or descriptive effects) of thermodynamic information.

Original languageEnglish
JournalDatabase : the journal of biological databases and curation
Volume2018
Issue number2018
DOIs
StatePublished - Jan 1 2018

Funding

National Natural Science Foundation of China (Nos. 21403002, 31601074, 11626052). Funding for open access charge: National Natural Science Foundation of China (No. 21403002).

FundersFunder number
National Natural Science Foundation of China11626052, 31601074, 21403002

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