Cytogenetic analysis of Populus trichocarpa - Ribosomal DNA, telomere repeat sequence, and marker-selected BACs

M. N. Islam-Faridi; C. D. Nelson; S. P. DiFazio; L. E. Gunter; G. A. Tuskan

doi:10.1159/000218749

Cytogenetic analysis of Populus trichocarpa - Ribosomal DNA, telomere repeat sequence, and marker-selected BACs

M. N. Islam-Faridi, C. D. Nelson, S. P. DiFazio, L. E. Gunter, G. A. Tuskan

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31 Scopus citations

Abstract

The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 18S-28S rDNA sites and one 5S rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.

Original language	English
Pages (from-to)	74-80
Number of pages	7
Journal	Cytogenetic and Genome Research
Volume	125
Issue number	1
DOIs	https://doi.org/10.1159/000218749
State	Published - Jul 2009

Keywords

BAC
FISH
Populus trichocarpa
Telomere
rDNA

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10.1159/000218749

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abstract = "The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 18S-28S rDNA sites and one 5S rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.",

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AU - Islam-Faridi, M. N.

AU - Nelson, C. D.

AU - DiFazio, S. P.

AU - Gunter, L. E.

AU - Tuskan, G. A.

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Y1 - 2009/7

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AB - The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization (FISH). Two 18S-28S rDNA sites and one 5S rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species.

KW - BAC

KW - FISH

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KW - Telomere

KW - rDNA

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Cytogenetic analysis of Populus trichocarpa - Ribosomal DNA, telomere repeat sequence, and marker-selected BACs

Abstract

Keywords

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