Crystallization and preliminary X-ray diffraction analysis of red clover necrotic mosaic virus

Stanton L. Martin; Richard H. Guenther; Tim L. Sit; Paul D. Swartz; Flora Meilleur; Steven A. Lommel; Robert B. Rose

doi:10.1107/S1744309110032483

Crystallization and preliminary X-ray diffraction analysis of red clover necrotic mosaic virus

Stanton L. Martin, Richard H. Guenther, Tim L. Sit, Paul D. Swartz, Flora Meilleur, Steven A. Lommel, Robert B. Rose

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Red clover necrotic mosaic virus (RCNMV) is a species that belongs to the Tombusviridae family of plant viruses with a T = 3 icosahedral capsid. RCNMV virions were purified and were crystallized for X-ray analysis using the hanging-drop vapor-diffusion method. Self-rotation functions and systematic absences identified the space group as I23, with two virions in the unit cell. The crystals diffracted to better than 4 Å resolution but were very radiation-sensitive, causing rapid decay of the high-resolution reflections. The data were processed to 6 Å in the analysis presented here.

Original language	English
Pages (from-to)	1458-1462
Number of pages	5
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	66
Issue number	11
DOIs	https://doi.org/10.1107/S1744309110032483
State	Published - Nov 2010
Externally published	Yes

Keywords

dianthoviruses
red clover necrotic mosaic virus
viruses

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10.1107/S1744309110032483

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title = "Crystallization and preliminary X-ray diffraction analysis of red clover necrotic mosaic virus",

abstract = "Red clover necrotic mosaic virus (RCNMV) is a species that belongs to the Tombusviridae family of plant viruses with a T = 3 icosahedral capsid. RCNMV virions were purified and were crystallized for X-ray analysis using the hanging-drop vapor-diffusion method. Self-rotation functions and systematic absences identified the space group as I23, with two virions in the unit cell. The crystals diffracted to better than 4 {\AA} resolution but were very radiation-sensitive, causing rapid decay of the high-resolution reflections. The data were processed to 6 {\AA} in the analysis presented here.",

keywords = "dianthoviruses, red clover necrotic mosaic virus, viruses",

author = "Martin, {Stanton L.} and Guenther, {Richard H.} and Sit, {Tim L.} and Swartz, {Paul D.} and Flora Meilleur and Lommel, {Steven A.} and Rose, {Robert B.}",

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T1 - Crystallization and preliminary X-ray diffraction analysis of red clover necrotic mosaic virus

AU - Martin, Stanton L.

AU - Guenther, Richard H.

AU - Sit, Tim L.

AU - Swartz, Paul D.

AU - Meilleur, Flora

AU - Lommel, Steven A.

AU - Rose, Robert B.

PY - 2010/11

Y1 - 2010/11

N2 - Red clover necrotic mosaic virus (RCNMV) is a species that belongs to the Tombusviridae family of plant viruses with a T = 3 icosahedral capsid. RCNMV virions were purified and were crystallized for X-ray analysis using the hanging-drop vapor-diffusion method. Self-rotation functions and systematic absences identified the space group as I23, with two virions in the unit cell. The crystals diffracted to better than 4 Å resolution but were very radiation-sensitive, causing rapid decay of the high-resolution reflections. The data were processed to 6 Å in the analysis presented here.

AB - Red clover necrotic mosaic virus (RCNMV) is a species that belongs to the Tombusviridae family of plant viruses with a T = 3 icosahedral capsid. RCNMV virions were purified and were crystallized for X-ray analysis using the hanging-drop vapor-diffusion method. Self-rotation functions and systematic absences identified the space group as I23, with two virions in the unit cell. The crystals diffracted to better than 4 Å resolution but were very radiation-sensitive, causing rapid decay of the high-resolution reflections. The data were processed to 6 Å in the analysis presented here.

KW - dianthoviruses

KW - red clover necrotic mosaic virus

KW - viruses

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SN - 1744-3091

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JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 11

ER -

Crystallization and preliminary X-ray diffraction analysis of red clover necrotic mosaic virus

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