CRISPR-based tools for microbial cell factories

Rongming Liu; Liya Liang; Sean Stettner; Emily F. Freed; Carrie A. Eckert

doi:10.1016/B978-0-12-821477-0.00001-5

CRISPR-based tools for microbial cell factories

Rongming Liu, Liya Liang, Sean Stettner, Emily F. Freed, Carrie A. Eckert

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

Abstract

The development of microbial chassis for the production of a variety of biochemicals and biofuels is a growing area of research. How to efficiently manipulate genetic information to achieve optimal production of these compounds is a key area of focus in the field. In recent years, clustered regularly interspaced palindromic repeats (CRISPR) and its associated proteins (Cas) have become a popular strategy for gene editing and regulation in many organisms due to its versatility and efficacy. Here, we describe methods developed utilizing CRISPR-Cas systems for engineering microbial cell factories (e.g., bacteria and yeast) at the single gene to genome scale for mutagenesis and transcriptional regulation of target genes. Finally, we provide a perspective on the challenges and opportunities for the applications of advanced CRISPR-Cas-based tools for engineering microbial cell factories.

Original language	English
Title of host publication	Microbial Cell Factories Engineering for Production of Biomolecules
Publisher	Elsevier
Pages	95-113
Number of pages	19
ISBN (Electronic)	9780128214770
DOIs	https://doi.org/10.1016/B978-0-12-821477-0.00001-5
State	Published - Jan 1 2021
Externally published	Yes

Keywords

CRIPSRa
CRISPR-Cas
CRISPRi
Gene editing
Genome editing

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/B978-0-12-821477-0.00001-5

Cite this

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abstract = "The development of microbial chassis for the production of a variety of biochemicals and biofuels is a growing area of research. How to efficiently manipulate genetic information to achieve optimal production of these compounds is a key area of focus in the field. In recent years, clustered regularly interspaced palindromic repeats (CRISPR) and its associated proteins (Cas) have become a popular strategy for gene editing and regulation in many organisms due to its versatility and efficacy. Here, we describe methods developed utilizing CRISPR-Cas systems for engineering microbial cell factories (e.g., bacteria and yeast) at the single gene to genome scale for mutagenesis and transcriptional regulation of target genes. Finally, we provide a perspective on the challenges and opportunities for the applications of advanced CRISPR-Cas-based tools for engineering microbial cell factories.",

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AU - Liu, Rongming

AU - Liang, Liya

AU - Stettner, Sean

AU - Freed, Emily F.

AU - Eckert, Carrie A.

PY - 2021/1/1

Y1 - 2021/1/1

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AB - The development of microbial chassis for the production of a variety of biochemicals and biofuels is a growing area of research. How to efficiently manipulate genetic information to achieve optimal production of these compounds is a key area of focus in the field. In recent years, clustered regularly interspaced palindromic repeats (CRISPR) and its associated proteins (Cas) have become a popular strategy for gene editing and regulation in many organisms due to its versatility and efficacy. Here, we describe methods developed utilizing CRISPR-Cas systems for engineering microbial cell factories (e.g., bacteria and yeast) at the single gene to genome scale for mutagenesis and transcriptional regulation of target genes. Finally, we provide a perspective on the challenges and opportunities for the applications of advanced CRISPR-Cas-based tools for engineering microbial cell factories.

KW - CRIPSRa

KW - CRISPR-Cas

KW - CRISPRi

KW - Gene editing

KW - Genome editing

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M3 - Chapter

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BT - Microbial Cell Factories Engineering for Production of Biomolecules

PB - Elsevier

ER -

CRISPR-based tools for microbial cell factories

Abstract

Keywords

UN SDGs

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