CP/MAS 13C NMR analysis of cellulase treated bleached softwood kraft pulp

Yunqiao Pu; Cherie Ziemer; Arthur J. Ragauskas

doi:10.1016/j.carres.2005.12.012

CP/MAS ¹³C NMR analysis of cellulase treated bleached softwood kraft pulp

Yunqiao Pu
, Cherie Ziemer
, Arthur J. Ragauskas

Research output: Contribution to journal › Article › peer-review

96 Scopus citations

Abstract

Fully bleached softwood kraft pulps were hydrolyzed with cellulase (1,4-(1,3:1,4)-β-D-glucan 4-glucano-hydrolase, EC 3.2.1.4) from Trichoderma reesei. Supra-molecular structural features of cellulose during enzymatic hydrolysis were examined by using CP/MAS ¹³C NMR spectra in combination with line-fitting analysis. Different types of cellulose allomorphs (cellulose I_α, cellulose I_β, para-crystalline) and amorphous regions were hydrolyzed to a different extent by the enzyme used. Also observed was a rapid initial phase for hydrolysis of regions followed by a slow hydrolysis phase. Cellulose I_α, para-crystalline, and non-crystalline regions of cellulose are more susceptible to enzymatic hydrolysis than cellulose I_β during the initial phase. After the initial phase, all the regions are then similarly susceptible to enzymatic hydrolysis.

Original language	English
Pages (from-to)	591-597
Number of pages	7
Journal	Carbohydrate Research
Volume	341
Issue number	5
DOIs	https://doi.org/10.1016/j.carres.2005.12.012
State	Published - Apr 10 2006
Externally published	Yes

Funding

The authors would like to gratefully acknowledge ARO (contract #: DAAD19-03-1-0244) for support of this study. In addition, we wish to thank the reviewers for their insightful comments.

Keywords

Cellulase hydrolysis
Cellulose
Crystallinity
NMR
Supra-molecular structure

Access to Document

10.1016/j.carres.2005.12.012

Cite this

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title = "CP/MAS 13C NMR analysis of cellulase treated bleached softwood kraft pulp",

abstract = "Fully bleached softwood kraft pulps were hydrolyzed with cellulase (1,4-(1,3:1,4)-β-D-glucan 4-glucano-hydrolase, EC 3.2.1.4) from Trichoderma reesei. Supra-molecular structural features of cellulose during enzymatic hydrolysis were examined by using CP/MAS 13C NMR spectra in combination with line-fitting analysis. Different types of cellulose allomorphs (cellulose Iα, cellulose Iβ, para-crystalline) and amorphous regions were hydrolyzed to a different extent by the enzyme used. Also observed was a rapid initial phase for hydrolysis of regions followed by a slow hydrolysis phase. Cellulose Iα, para-crystalline, and non-crystalline regions of cellulose are more susceptible to enzymatic hydrolysis than cellulose Iβ during the initial phase. After the initial phase, all the regions are then similarly susceptible to enzymatic hydrolysis.",

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AU - Ragauskas, Arthur J.

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