Covalent Attachment of Gold Nanoparticles to DNA Templates

Karen A. Stevenson; G. Muralidharan; Leon Maya; Jack C. Wells; Jacob Barhen; Thomas Thundat

doi:10.1166/jnn.2002.110

Covalent Attachment of Gold Nanoparticles to DNA Templates

Karen A. Stevenson, G. Muralidharan, Leon Maya, Jack C. Wells, Jacob Barhen, Thomas Thundat

Metals and Composites Processing

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Functionalized gold nanoparticles have been covalently bound to internal, modified sites on double-stranded DNA. Gold nanoparticles coated with mercaptosuccinic acid or thioctic acid were bound to amino-modified thymine bases on double-stranded DNA. Visible absorption spectra, gel electrophoresis, and atomic force microscopy were used to analyze the products. Thiol groups were added to one end of the gold/nanoparticle product, which was then attached to a gold surface. This method has the potential to allow controlled placement of particles with subnanometer precision and to allow attachment of the product to fixed contacts for nanodevice fabrication.

Original language	English
Pages (from-to)	397-404
Number of pages	8
Journal	Journal of Nanoscience and Nanotechnology
Volume	2
Issue number	3-4
DOIs	https://doi.org/10.1166/jnn.2002.110
State	Published - Jun 2002

Keywords

Absorption Spectroscopy
Atomic Force Microscopy
DNA Templates
Gel Electrophoresis
Gold Nanoparticles
Oligonucleotides

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10.1166/jnn.2002.110

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abstract = "Functionalized gold nanoparticles have been covalently bound to internal, modified sites on double-stranded DNA. Gold nanoparticles coated with mercaptosuccinic acid or thioctic acid were bound to amino-modified thymine bases on double-stranded DNA. Visible absorption spectra, gel electrophoresis, and atomic force microscopy were used to analyze the products. Thiol groups were added to one end of the gold/nanoparticle product, which was then attached to a gold surface. This method has the potential to allow controlled placement of particles with subnanometer precision and to allow attachment of the product to fixed contacts for nanodevice fabrication.",

keywords = "Absorption Spectroscopy, Atomic Force Microscopy, DNA Templates, Gel Electrophoresis, Gold Nanoparticles, Oligonucleotides",

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AU - Stevenson, Karen A.

AU - Muralidharan, G.

AU - Maya, Leon

AU - Wells, Jack C.

AU - Barhen, Jacob

AU - Thundat, Thomas

PY - 2002/6

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AB - Functionalized gold nanoparticles have been covalently bound to internal, modified sites on double-stranded DNA. Gold nanoparticles coated with mercaptosuccinic acid or thioctic acid were bound to amino-modified thymine bases on double-stranded DNA. Visible absorption spectra, gel electrophoresis, and atomic force microscopy were used to analyze the products. Thiol groups were added to one end of the gold/nanoparticle product, which was then attached to a gold surface. This method has the potential to allow controlled placement of particles with subnanometer precision and to allow attachment of the product to fixed contacts for nanodevice fabrication.

KW - Absorption Spectroscopy

KW - Atomic Force Microscopy

KW - DNA Templates

KW - Gel Electrophoresis

KW - Gold Nanoparticles

KW - Oligonucleotides

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JO - Journal of Nanoscience and Nanotechnology

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ER -

Covalent Attachment of Gold Nanoparticles to DNA Templates

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Keywords

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