Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion

Zhi Min Zhao; Xianzhi Meng; Brent Scheidemantle; Yunqiao Pu; Zhi Hua Liu; Bing Zhi Li; Charles E. Wyman; Charles M. Cai; Arthur J. Ragauskas

doi:10.1016/j.biortech.2021.126367

Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion

Zhi Min Zhao, Xianzhi Meng, Brent Scheidemantle, Yunqiao Pu, Zhi Hua Liu, Bing Zhi Li, Charles E. Wyman, Charles M. Cai, Arthur J. Ragauskas

Biomaterials and Biomass Characterizatio

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Cosolvent Enhanced Lignocellulosic Fractionation (CELF) is an emerging solvolysis pretreatment to fractionate lignocellulosic biomass. Herein, the bioconversion performance of CELF lignin was fully evaluated for the first time. Results showed that CELF lignin possessed higher content of carboxylic acid OH, lower molecular weight, and disappeared β-O-4 and β-5 linkages compared to other two technical lignins including a conventional ethanol organosolv lignin (EOL) and a kraft lignin (KL). Rhodococcus opacus PD630 cell count from CELF lignin fermentation reached the highest value of 3.9 × 10⁷ CFU/mL, representing a 62.5% and 77.3% improvement over EOL and KL, respectively. Correspondingly, lipid yield reached 143 mg/L from CELF lignin, which was 36.2% and 26.5% higher than from EOL and KL, respectively. Principal component analysis (PCA) revealed that more carboxylic acid groups and lower molecular weight contributed to the enhanced bioconversion performance of CELF lignin. This study demonstrates that CELF lignin is a promising candidate for bioconversion.

Original language	English
Article number	126367
Journal	Bioresource Technology
Volume	347
DOIs	https://doi.org/10.1016/j.biortech.2021.126367
State	Published - Mar 2022

Funding

ZMZ acknowledges the National Natural Science Foundation of China (grant numbers 21706136, 21766025) and the China Scholarship Council (CSC) for their support. In addition, AJR, CMC, CEW, YP acknowledge the partial support from the Center for Bioenergy Innovation (CBI), a U.S. Department of Energy (DOE) Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. Oak Ridge National Laboratory is managed by UT-Battelle, LLC under Contract DE-AC05-00OR22725 with the U.S. DOE.

Funders	Funder number
U.S. Department of Energy
Office of Science
Biological and Environmental Research
Oak Ridge National Laboratory
Center for Bioenergy Innovation
UT-Battelle	DE-AC05-00OR22725
National Natural Science Foundation of China	21706136, 21766025
China Scholarship Council

Keywords

Bioconversion
Cosolvent Enhanced Lignocellulosic Fractionation (CELF)
Lignin valorization
Rhodococcus opacus PD630

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10.1016/j.biortech.2021.126367

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title = "Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion",

abstract = "Cosolvent Enhanced Lignocellulosic Fractionation (CELF) is an emerging solvolysis pretreatment to fractionate lignocellulosic biomass. Herein, the bioconversion performance of CELF lignin was fully evaluated for the first time. Results showed that CELF lignin possessed higher content of carboxylic acid OH, lower molecular weight, and disappeared β-O-4 and β-5 linkages compared to other two technical lignins including a conventional ethanol organosolv lignin (EOL) and a kraft lignin (KL). Rhodococcus opacus PD630 cell count from CELF lignin fermentation reached the highest value of 3.9 × 107 CFU/mL, representing a 62.5% and 77.3% improvement over EOL and KL, respectively. Correspondingly, lipid yield reached 143 mg/L from CELF lignin, which was 36.2% and 26.5% higher than from EOL and KL, respectively. Principal component analysis (PCA) revealed that more carboxylic acid groups and lower molecular weight contributed to the enhanced bioconversion performance of CELF lignin. This study demonstrates that CELF lignin is a promising candidate for bioconversion.",

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author = "Zhao, {Zhi Min} and Xianzhi Meng and Brent Scheidemantle and Yunqiao Pu and Liu, {Zhi Hua} and Li, {Bing Zhi} and Wyman, {Charles E.} and Cai, {Charles M.} and Ragauskas, {Arthur J.}",

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AU - Zhao, Zhi Min

AU - Meng, Xianzhi

AU - Scheidemantle, Brent

AU - Pu, Yunqiao

AU - Liu, Zhi Hua

AU - Li, Bing Zhi

AU - Wyman, Charles E.

AU - Cai, Charles M.

AU - Ragauskas, Arthur J.

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AB - Cosolvent Enhanced Lignocellulosic Fractionation (CELF) is an emerging solvolysis pretreatment to fractionate lignocellulosic biomass. Herein, the bioconversion performance of CELF lignin was fully evaluated for the first time. Results showed that CELF lignin possessed higher content of carboxylic acid OH, lower molecular weight, and disappeared β-O-4 and β-5 linkages compared to other two technical lignins including a conventional ethanol organosolv lignin (EOL) and a kraft lignin (KL). Rhodococcus opacus PD630 cell count from CELF lignin fermentation reached the highest value of 3.9 × 107 CFU/mL, representing a 62.5% and 77.3% improvement over EOL and KL, respectively. Correspondingly, lipid yield reached 143 mg/L from CELF lignin, which was 36.2% and 26.5% higher than from EOL and KL, respectively. Principal component analysis (PCA) revealed that more carboxylic acid groups and lower molecular weight contributed to the enhanced bioconversion performance of CELF lignin. This study demonstrates that CELF lignin is a promising candidate for bioconversion.

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Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion

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