COP9 signalosome subunit 5A affects phenylpropanoid metabolism, trichome formation and transcription of key genes of a regulatory tri-protein complex in Arabidopsis

Shu Wei, Xiang Li, Margaret Y. Gruber, Biruk A. Feyissa, Lisa Amyot, Abdelali Hannoufa

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Background: Trichomes and phenylpropanoid-derived phenolics are structural and chemical protection against many adverse conditions. Their production is regulated by a network that includes a TTG1/bHLH/MYB tri-protein complex in Arabidopsis. CSN5a, encoding COP9 signalosome subunit 5a, has also been implicated in trichome and anthocyanin production; however, the regulatory roles of CSN5a in the processes through interaction with the tri-protein complex has yet to be investigated. Results: In this study, a new csn5a mutant, sk372, was recovered based on its altered morphological and chemical phenotypes compared to wild-type control. Mutant characterization was conducted with an emphasis on trichome and phenylpropanoid production and possible involvement of the tri-protein complex using metabolite and gene transcription profiling and scanning electron microscopy. Seed metabolite analysis revealed that defective CSN5a led to an enhanced production of many compounds in addition to anthocyanin, most notably phenylpropanoids and carotenoids as well as a glycoside of zeatin. Consistent changes in carotenoids and anthocyanin were also found in the sk372 leaves. In addition, 370 genes were differentially expressed in 10-day old seedlings of sk372 compared to its wild type control. Real-time transcript quantitative analysis showed that in sk372, GL2 and tri-protein complex gene TT2 was significantly suppressed (p < 0.05) while complex genes EGL3 and GL3 slightly decreased (p > 0.05). Complex genes MYB75, GL1 and flavonoid biosynthetic genes TT3 and TT18 in sk372 were all significantly enhanced. Overexpression of GL3 driven by cauliflower mosaic virus 35S promotor increased the number of single pointed trichomes only, no other phenotypic recovery in sk372. Conclusions: Our results indicated clearly that COP9 signalosome subunit CSN5a affects trichome production and the metabolism of a wide range of phenylpropanoid and carotenoid compounds. Enhanced anthocyanin accumulation and reduced trichome production were related to the enhanced MYB75 and suppressed GL2 and some other differentially expressed genes associated with the TTG1/bHLH/MYB complexes.

Original languageEnglish
Article number134
JournalBMC Plant Biology
Volume18
Issue number1
DOIs
StatePublished - Jun 25 2018
Externally publishedYes

Funding

Authors acknowledged the technical help from Drs. Guo-Feng Liu and Fu-Ming Wang at the Anhui Agricultural University for data analysis and growth chamber management of sk372. National Natural Science Foundation of China (Award numbers 31770734 and 31370687).

FundersFunder number
National Natural Science Foundation of China31770734, 31370687
Anhui Agricultural University

    Keywords

    • Anthocyanin
    • Arabidopsis
    • COP9 signalosome
    • CSN5a
    • MYB75
    • Trichomes

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